For automated isolation of viral RNA from cell-free body fluids
Fast setup with preloaded rotor adapters
Standardized results and streamlined workflow
Helps to eliminate loading errors
Automated purification, with up to 12 samples per run
The QIAamp Viral RNA Mini QIAcube Kit is a highly convenient format of the QIAamp Viral RNA Mini Kit, dedicated for use on the QIAcube. The QIAamp Viral RNA Mini QIAcube Kit provides rapid isolation of high-quality, ready-to-use RNA from cell-free body fluids.
For 240 viral RNA minipreps: QIAamp Mini Rotor Adapters (preloaded with spin columns and elution tubes); carrier RNA and RNase-free reagents and buffers.
The high-quality viral RNA isolated using the QIAamp Viral RNA Mini QIAcube Kit performs well in a wide range of downstream applications, including viral genotyping, viral epidemiology, and infectious disease research.
The QIAamp Viral RNA Mini QIAcube Kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings (see figure "Significant time savings"). Furthermore, ease of use is increased and user errors minimized. Waste is reduced, because the content of the dedicated kit is tailored for purification on the QIAcube and the superfluous tubes that are required for the manual procedure are not included.
The QIAamp Viral RNA Mini QIAcube Kit simplifies isolation of viral RNA from cell-free body fluids. No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.
The award-winning QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run. The QIAcube together with the dedicated QIAamp Viral RNA Mini QIAcube Kit provides a winning combination for fast, easy, and convenient viral RNA purification.
The QIAamp Viral RNA Mini QIAcube Kit is for automated purification of viral RNA from cell-free body fluids on the QIAcube. Sample sources include:
For 100 x 50 µl reactions: QIAGEN OneStep RT-PCR Enzyme Mix (1 x 200 µl), 5x QIAGEN OneStep RT-PCR Buffer (1 x 1 ml), dNTP Mix (1 x 200 µl, 10 mM each), 5x Q-Solution (1 x 2 ml), RNase-Free Water (2 x 1.9 ml)
For 100 x 20 µl reactions: 1 x 500 µl QuantiNova Pathogen Master Mix, 1 x 500 µl Yellow Template Dilution Buffer, 1 x 250 µl ROX Reference Dye, 1 x 1.9 µl RNase-Free Water, 1 x 1500 µl Nucleic Acid Dilution Buffer, 1 x 100 µl Internal Control RNA, 1 x 100 µl Internal Control DNA, 1 x 200 µl IC Probe Assay