QIAsymphony DNA Maxi Kit

Automate the purification of high-quality gDNA from human whole blood samples (up to 4 ml) using the QIAsymphony SP.

Automated DNA purification from 4 ml on the QIAsymphony SP results in high yields (measured via  gel electrophoresis and  spectrophotometer) of high-molecular-weight DNA (see  High-molecular-weight DNA) in 4.75 hours (see  QIAsymphony DNA Maxi Kit workflow), and even faster for 2 ml or for fewer than 24 samples.

The QIAsymphony DNA Maxi Kit uses an interlaced system (see  QIAsymphony DNA Maxi Kit workflow) that shortens the time needed to complete the process:

1. The QIAsymphony SP takes 1 ml from the 4 ml sample and lyses that portion.
2. While the first 1 ml is in the bind process, a second 1 ml is taken from the sample and gets lysed simultaneously.
3. DNA from this 1 ml is bound with the same magnetic beads that were used for the first 1 ml. While this is happening, the third 1 ml is being lysed.
4. DNA from the third 1 ml is bound in the same magnetic beads used with the previous portions. At the same time, the fourth 1 ml is being lysed.
5. DNA from the last 1 ml portion is bound like the others.

The QIAsymphony SP performs all 4 steps (lyse, bind, wash and elute) of the purification procedure for up to 36 samples in a single run.