For purification of up to 600 µg genomic, mitochondrial, or viral DNA from blood
Rapid purification of high-quality, ready-to-use DNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors
The QIAamp DNA Blood Maxi Kit provides silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Maxi Kit is designed for processing 3–10 ml fresh or frozen human whole blood. The QIAamp Maxi spin columns can be easily processed in a centrifuge or on vacuum manifolds (Additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with the vacuum manifolds).
For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
QIAamp Spin Column procedure.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.
Apoptotic banding in stored blood.
Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1: lambda-HindIII; M2:100 bp ladder.
Amplification of a 10 kb fragment of the human ALDH1 gene from genomic DNA isolated from blood. DNA was purified using conventional methods (Phenol) or the QIAamp DNA Blood Maxi Kit (QIAamp). PCR products were sequenced directly. M: 1 kb ladder.
Paternity testing by RFLP analysis.
Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg of DNA loaded. Outcome: A: alleged father excluded; B & C: alleged father confirmed. (Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)
QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table).
DNA yields from human whole blood with different cell densities.
Leukocytes per ml
DNA yield (µg)
DNA yield (µg)
2.5 x 105
1.0 x 106
5.0 x 106
1.0 x 107
Using the QIAamp DNA Blood Maxi Kit, genomic DNA was purified from 10 ml of human whole blood and eluted in 1 ml elution buffer. The first eluate was loaded onto the column a second time and centrifuged again (i.e., re-eluted). Percentage DNA recovery was calculated by assuming that one leukocyte contains 6.6 pg DNA.
No phenol–chloroform extraction is required. The DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit.
The QIAamp DNA Blood Maxi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Maxi Kit processes sample sizes of 3–10 ml, with a preparation time of 55 minutes. The typical yield from 10 ml healthy whole blood is 300–600 µg, with an elution volume of 500–2000 µl.
The QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources. QIAamp sample preparation technology is fully licensed.
The QIAamp DNA Blood Maxi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Maxi spin columns with 50 ml collection tubes. Sample sources include:
Fresh and frozen whole blood or buffy coat
Plasma or serum
Main sample type
Whole blood, body fluids
Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.