RT2 Profiler PCR Array Service
For pathway- or disease-focused gene expression profiling
The RT2 Profiler PCR Array Service uses RT2 Profiler PCR Arrays, a robust qRT-PCR technology that simultaneously monitors the expression of 84 or 370 pathway-focused genes. Verified and optimized processes yield outstanding data quality and rapid turnaround time.
The RT2 Profiler PCR Array Service is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The RT2 Profiler PCR Array Service uses high-performance SYBR® Green real-time PCR analysis. RT² Profiler PCR Arrays analyze expression of a panel of genes associated with any one of over 120 biological pathways or specific disease states. RT² Profiler PCR Arrays provide accurate, reliable, and convenient SYBR® Green analysis. Choose from over 120 catalogued arrays or custom design an array of your choice.
Recommended sample types and sample collection details are described below.
Provide at least 0.5 million (106) cells for each sample (specify number of cells on the submission form). Snap freeze the cell pellets in liquid nitrogen, store samples frozen at –80°C, and ship on dry ice.
Provide at least 50 mg of tissue for each sample (specify the weight of the tissue on the submission form). Snap freeze tissues in liquid nitrogen. Store samples frozen at –80°C, and ship on dry ice.
Where total RNA samples are submitted, a minimum concentration of 0.5 mg/ml is required. If the RNA concentration is below 0.5 mg/ml, please contact QIAGEN Technical Services. A260/A280 should be >2.0 and A260/A230 should be >1.7. All measurements should be taken in RNase-free 10 mM Tris buffer; pH 8.0.
If the submitted samples are cells or tissue, RNA extraction can be performed using the RNA Isolation Service.
Other sample types
Please contact QIAGEN Technical Services.
RNA samples are processed by QIAGEN for RT2 Profiler PCR Arrays using the following steps:
Data analysis is performed using dedicated PCR array data analysis software. Data in an electronic form can be downloaded from a secure FTP website or delivered via email. A complete data and experiment report includes:
Contact a Technical Support representative to order or to discuss your specific need at firstname.lastname@example.org.
RT2 Profiler PCR arrays are the most reliable and sensitive profiling technology for analyzing a panel of genes in signal transduction pathways, biological processes or disease-related gene networks. RT2 Profiler PCR arrays can be used for research into cancer, immunology, stem cells, toxicology, biomarker discovery and verification, and phenotypic analysis of cells, tissues, and research animal samples.
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