For the isolation of total RNA from stool and gut material
Fast and easy protocol isolates high-quality total RNA in less than 45 minutes
Efficient isolation of total RNA from difficult microbiome samples
Highly pure RNA isolation with Inhibitor Removal Technology
Simple co-isolation of miRNA and DNA using an on-column procedure
Get high-quality RNA fast, free of lingering PCR inhibitors with the RNeasy PowerMicrobiome Kit. Designed for purification of total RNA from stool, dried feces, gut material, contaminated buccal swabs and secretions, the RNeasy Power Microbiome Kit isolates RNA that is ready for demanding downstream applications including RT-PCR, qPCR and RNA-seq. Digested food, heme from lysed red blood cells in stool and other PCR inhibitors are no match for the Inhibitor Removal Technology that is included in the kit. The RNeasy PowerMicrobiome Kit also isolates miRNA and can optionally co-isolate DNA using a simple, on-column procedure using reagents provided with the kit.
RNeasy PowerMicrobiome Kit was formerly sold by MO BIO as PowerMicrobiome RNA Isolation Kit.
The RNeasy PowerMicrobiome Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Isolation of high quality, intact total RNA from human stool using the RNeasy PowerMicrobiome Kit. Samples were collected from a healthy volunteer and total RNA was purified using the DNeasy PowerMicrobiome Kit. An Agilent Bioanalyzer RNA Nano kit chip was used to profile and quantify 1 µl of RNA. Clear 16S and 23S bands for prokaryotic rRNA were observed in the electrophoresis image (left) and are shown in detail in the electropherogram (right). High quality RNA was observed, as demonstrated by a RIN of 9.4. Data courtesy of C. Vincent, McGill University, Montréal (Québec) Canada.
Isolation of high quality total RNA from stool samples using the RNeasy PowerMicrobiome Kit. Fresh canine stool samples were collected in an experimental RNA preservation solution and transported to the lab at room temperature (lanes 2-3) or on ice (lanes 4-5). Sample collection and processing were performed on the same day. Total RNA was isolated using the RNeasy PowerMicrobiome Kit and 10 µl of each sample was examined on a 1% agarose gel.
0.1 mm glass
Up to 40 µg per prep
Silica Spin Filter Tubes
Processed stool, gut material, dried feces, contaminated buccal swabs and secretionsStool, gut material, dried feces, contaminated buccal swabs and secretions
Store lyophilized DNase I at 4°C All other kit reagents and components should be stored at room temperature (15-30°C)