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QIAamp MinElute Virus Vacuum Kit

For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids

  • Rapid purification of high-quality viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp MinElute Virus Vacuum Kit simplifies purification of viral DNA and RNA with fast vacuum procedures. The QIAamp MinElute Virus Vacuum Kit can be used with starting sample volumes up to 0.5 ml. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl.

Cat No./ID: 57714
QIAamp MinElute Virus Vacuum Kit (50)
€273.00
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For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)
The QIAamp MinElute Virus Vacuum Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAamp MinElute Virus Vacuum procedure.
Performance

Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").

Viral nucleic acids purified using the QIAamp MinElute Virus Vacuum Kit can be used in a wide range of downstream applications, including:

PCR and quantitative real-time PCR
Infectious disease research
Principle

The QIAamp MinElute Virus Vacuum Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids that are ready to use in PCR and blotting procedures.

The QIAamp MinElute Virus Vacuum Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. QIAamp sample preparation technology is fully licensed.

Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "Procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Applications

The QIAamp MinElute Virus Vacuum Kit uses well-established technology for simultaneous purification of viral RNA and DNA. Fresh or frozen plasma, serum, and other cell-free body fluids (500 µl) can be processed in less than an hour.

Features
Specifications
Applications PCR, real-time PCR
Elution volume 20–150 µl
Format MinElute columns
Main sample type Serum, plasma
Processing Manual (vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 500 µl
Technology Silica technology
Time per run or per prep <1 hour
Yield Varies

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Kit Handbooks (2)
For simultaneous purification of viral RNA and DNA from plasma, serum, and cell-free body fluids
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血漿、血清、無細胞体液からのウィルス核酸を高濃度で精製 (吸引法)  
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User-Developed Protocols (1)
This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.
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