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PAXgene Blood miRNA Kit

For purification of miRNA and total RNA from whole blood

  • Integrated system for collection, stabilization, and purification
  • Effective purification of miRNA and total RNA
  • RNA stabilization for up to 3 days at 18–25°C
  • Stabilization for at least 50 months at –20°C or –70°C

The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.

Cat No./ID: 763134
PAXgene Blood miRNA Kit (50)
€641.00
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For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Analysis of miRNA species.
Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to–30°C. RNA was purified using the PAXgene Blood RNA Kit or the PAXgene Blood miRNA Kit, as indicated. miRNA species were analyzed using the QIAGEN miScript PCR System, with the indicated miScript Primer Assays. The lower CT values for RNA purified using the PAXgene Blood miRNA Kit indicate higher amounts of those miRNA species.
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High yields of small RNA species.
Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to –30°C. RNA was purified using the PAXgene Blood RNA Kit (RNA kit) or the PAXgene Blood miRNA Kit (miRNA kit). [A] Purified RNA was analyzed by agarose gel electrophoresis. [B] Purified RNA was analyzed using the Agilent Small RNA LabChip. Small RNA species were significantly enriched when using the PAXgene Blood miRNA Kit.
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The QIAcube.
Samples can be processed manually or automated on the QIAcube.
Performance
Blood samples collected in PAXgene Blood RNA Tubes can be safely stored or transported at 15–25°C for up to 72 hours, at 2–8°C for up to 5 days, or at –20°C or –70°C for at  least 50 months without showing any significant RNA degradation or changes in transcript levels. Total RNA >18 nucleotides is purified from the stabilized blood samples with the PAXgene Blood miRNA Kit and includes both mRNA and small RNAs such as miRNA (see figure "High yields of small RNA species"). The miRNA species can be analyzed using the QIAGEN miScript PCR System (see figure "Analysis of miRNA species") or other real-time RT-PCR systems.
Principle

Blood samples are collected in PAXgene Blood RNA Tubes. The tubes contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn.

Procedure
With the PAXgene Blood miRNA Kit, total RNA >18 nucleotides (including miRNA) is purified from the stabilized blood samples using well-established PAXgene silica-membrane technology. Purification can be carried out in a manual format or it is automatable on the QIAcube (see figure "The QIAcube").
Applications

The purified total RNA is ready to use and is ideally suited for any downstream application, including:

RT-PCR and real-time RT-PCR
Differential display
cDNA synthesis
Northern, dot, and slot blot analyses
Primer extension
Poly A+ RNA selection
RNase/S1 nuclease protection
Microarray analysis
Features
Specifications
Applications RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis
Elution volume 40 µl
Format Spin column
Main sample type Whole blood
Processing Manual (centrifugation) or on QIAcube
Sample amount 2.5 ml
Technology Silica technology
Time per run or per prep 90 minutes/12 samples
Yield >3 µg (total RNA)

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Brochures & Guides (2)

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For purification of intracellular RNA, including miRNA, from whole blood

 


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Kit Handbooks (1)
For manual or automated purification of miRNA from whole blood
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Application Notes (2)
Comparison of miRNA recovery from the PAXgene Blood miRNA System and the Tempus Blood RNA Tube processed with the Tempus Spin RNA Isolation Kit

 


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Typical total RNA yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood miRNA Kit
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