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Rotor-Disc 100 Starter Kit

For use with the Rotor-Gene Q
  • Accelerated reaction setup and high throughput
  • Outstanding performance in real-time PCR with the Rotor-Gene Q

The Rotor-Disc 100 Starter Kit includes all you need for runs of 100 reactions on the Rotor-Gene Q real-time cycler.

Cat No./ID: 9001603
Rotor-Disc 100 Starter Kit (230V)
€3,966.00
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3 Rotor-Disc 100 packs for 15–25 µl reaction volumes, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 100 Rotor and Locking Ring, Rotor-Disc Pipetting Aid, Rotor-Disc Easy Lift Adaptor

The Rotor-Disc 100 Starter Kit is for use with the Rotor-Gene Q thermal cycler.



Principle

The Rotor-Disc 100 Starter Kit enables easy start up of real-time PCR experiments on the Rotor-Gene Q using the Rotor-Disc 100 format. The kit includes Rotor-Disc 100 packs, a Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, a Rotor-Disc 100 Rotor and Locking Ring, a Rotor-Disc 100 Loading Block, and a Rotor-Disc Pipetting Aid.

Rotor-Discs are fully compatible with the QIAgility instrument, which enables automated reaction setup.

Procedure

The Rotor-Disc 100 Rotor and Rotor-Disc 100 Locking Ring are used with the Rotor-Disc 100. The Rotor-Disc 100 is a circular plate of 100 vertically oriented reaction wells for high-throughput use. The Rotor-Disc 100 is the rotary equivalent of a 96-well plate but with an additional 4 reference wells. It enables integration of the Rotor-Gene Q with 96-well laboratory workflows. The extra wells can be conveniently used for more samples, additional control reactions, or orientation reactions, without occupying any of the standard 96-well positions. For seamless 96-well workflow compatibility, Rotor-Disc 100 wells use 96-well plate labeling conventions, i.e., A1-A12 through to H1-H12. The additional 4 reference wells are labeled R1-R4.

The Rotor-Disc 100 does not use caps. Instead, Rotor-Disc Heat Sealing Film is applied to the top and heat sealed using a Rotor-Disc Heat Sealer. The film prevents contamination by providing a strong, durable, and tamper-proof seal.

Applications

The Rotor-Gene Q and Rotor-Disc 100 Starter Kit enable real-time PCR and high-resolution melting (HRM) applications, including:

  • Gene expression analysis
  • Pathogen detection
  • DNA methylation analysis
  • Genotyping
  • Gene scanning
  • miRNA research
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