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Qproteome Bacterial Protein Prep Kit

For preparation of soluble bacterial proteins
  • Gentle but effective cell lysis, high yields of active proteins
  • Simple procedure, no specialized equipment required
  • Highly reproducible procedure, consistent results time after time

The kit contains a lysis buffer for preparation of soluble proteins from bacterial cultures for use in proteomics procedures.

Cat No./ID: 37900
Qproteome Bacterial Protein Prep Kit
€202.00
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For soluble protein preparations from up to 4.5 liters of bacterial cell culture: Lysis Buffer, Lysozyme, Benzonase®
The Qproteome Bacterial Protein Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Principle

Bacterial cell walls are disrupted by the combination of a freeze–thaw cycle and a lysis buffer containing lysozyme. The lysis buffer also contains a nuclease, which reduces the viscosity of the lysate and frees proteins associated with nucleic acids. Once lysis is complete, the lysate is centrifuged to precipitate insoluble proteins and cell debris.

Procedure

Lysis buffer is added to frozen cell pellets, cells are resuspended, and incubated on ice for 30 minutes. The lysate is centrifuged to precipitate insoluble proteins and cell debris, and soluble proteins are collected in the supernatant.

Applications
The Qproteome Bacterial Protein Prep Kit is used for preparation of soluble bacterial proteins for proteomics procedures (e.g., 2D-PAGE, mass spectrometry).
Features
Specifications
Applications SDS-PAGE, mass spectrometry
Fractions isolated One fraction
Sample size Pellet from a 250 ml sample
Species Bacteria
Start material Cell cultures
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