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Qproteome Albumin/IgG Depletion Kit

For depletion of albumin and IgG from human serum and plasma samples
  • Efficient removal of human albumin and IgG
  • Highly specific depletion using immobilized monoclonal antibodies
  • Fast and easy-to-use spin column format

The kit contains spin columns for depletion of albumin and IgG from human serum or plasma samples. Spin Column Adapter Rings are required for automation of this kit on the QIAcube.

Cat No./ID: 37521
Qproteome Albumin/IgG Depletion Kit
€306.00
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For albumin/IgG depletion of 6 serum or plasma samples: Albumin/IgG Depletion Spin Columns (6)
The Qproteome Albumin/IgG Depletion Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Qproteome Albumin/IgG Depletion Kit procedure.
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Albumin/IgG depletion facilitates analysis of low-abundance proteins.
Coomassie stained 2D-PAGE gels showing [A] non-depleted and [B] depleted (right) plasma samples.
Performance
The Qproteome Albumin/IgG Depletion Kit provides efficient and specific removal of highly abundant albumin and IgG from human serum and plasma samples (see figure “Albumin/IgG depletion facilitates analysis of low-abundance proteins.")
Principle

Albumin and IgG in the sample are bound by antibodies immobilized on a solid support in the spin columns.

Procedure
Diluted samples are applied to the resin in the spin columns, which are then sealed and incubated on an end-over-end shaker. Plasma and serum proteins, depleted of albumin and IgG, are recovered by centrifugation (see "Qproteome Albumin/IgG Depletion Kit procedure").
Applications

The Qproteome Albumin/IgG Depletion Kit facilitates analysis of less abundant proteins.

Features
Specifications
Applications SDS-PAGE, mass spectrometry
Binding capacity/yield 0.5–0.8 mg
Fractions isolated One fraction
Sample size <25 µl
Species Human
Start material Serum, plasma
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