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NoviPure Soil Protein Kit

For the extraction of protein from all soil types
  • Efficient extraction of microbial protein from up to 5 grams of soil
  • Cleaner proteins with less humic substance interference
  • Improved downstream results with high-quality proteins
Get more proteins from your soil samples with less interference using the NoviPure Soil Protein Kit.

This novel kit extracts extracellular and intracellular microbial protein without co-extracting humic substances or other interfering compounds. The bead beating protocol uses a mixture of glass and ceramic beads along with two buffers to efficiently break down intracellular and extracellular protein. All materials are certified protein- and protease-free.

The resulting protein pellet has fewer impurities compared to other extraction methods and can be easily resuspended for further analysis or storage. Proteins are ready for 1D and 2D polyacrylamide gel electrophoresis and mass spectrometry (2D LC-MS/MS).

The NoviPure Soil Protein Kit was previously sold by MO BIO under the same name.
Cat No./ID: 30000-20
NoviPure Soil Protein Kit (20)
€617.00
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For the extraction of protein from all soil types
The NoviPure Soil Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1.
Cleaner protein is observed in compost samples extracted with NoviPure Soil Protein Kit. (A) Total protein was extracted from 5 g of sterile compost samples spiked witth C. albicans using either the NoviPure Soil Protein Kit (samples 1-2) or a method employing sucrose and phenol extractions1 (4, 5). A pure C. albicans culture was extracted with the NoviPure Soil Protein Kit (3) or sucrose/phenol as a control (6). (B) Total protein was extracted from 5 g sterile compost samples spiked with E. coli using either the NoviPure Soil Protein Kit (samples 1-2) or a method employing SDS and boiling2 (3, 4). Proteins (20 µl per sample) were run on a 1D SDS-PAGE gel and visualized using stain-free gels (3). In each case, samples extracted with the NoviPure Soil Protein Kit contained fewer humic substances as evidenced by reduced background smearing, making it possible to visualize protein bands on a 1D gel
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Figure 2.
High protein yields are observed from challenging soil types using the NoviPure Soil Protein Extraction Kit. Total protein was extracted from 5 g samples of sterile soil and sediments spiked with E. coli using the NoviPure Soil Protein Extraction Kit. Proteins (20 µl per sample) were run on a 1D SDS-PAGE gel and visualized using stain-free gels (3). Protein bands were easily visualized in all cases, and matched the E. coli culture control. (1, 2) - Freshwater lake sediment; (3, 4) - Lagoon sediment; (5, 6) - Beach sand; (7, 8) - Agricultural soil; (9, 10) - Pure E. coli culture.
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Figure 3.
Successful results in 2D Gel Electrophoresis using protein extracted with the NoviPure Soil Protein Kit. Protein was extracted from 5 g sterile compost samples spiked with E. coli using the NoviPure Soil Protein Kit. Protein (20 ul per sample), was analyzed by 2D SDS-PAGE in the pH range 3-10 and visualized using silver staining. Protein was easily visualized with no interference from humic substances.
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Figure 4.
Accurate results in 2D LC - MS/MS using protein extracted with the NoviPure Soil Protein Kit. Total ion chromatogram data showing base peaks of protein. Image is fraction 4 of SCX cation exchange. Protein was extracted from 5 g sterile compost samples spiked with E. coli using the NoviPure Soil Protein Kit. Proteins were digested and peptide enriched using reverse phase ion exchange and analyzed with 2D LC - MS/MS. Raw spectra were searched against SwissProt protein database (March 2013 release). 1076 unique proteins were identified.

1) Taylor & Williams, 2013 Microb. Ecol. 59:390-99

2) Chourey et al. 2010 J. Proteome Res. 9:6615-22

3) BIORAD mini PROTEAN TGX Stain-Free Gels
Performance


Features
Specifications
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