NeXtal CubicPhase System
For membrane protein crystallization
The NeXtal CubicPhase crystallization system enables fully automatable, high-throughput membrane protein crystallization setup using a standard liquid-handling robot or manual setup using a multichannel pipet. The NeXtal CubicPhase Crystallization System includes NeXtal CubicPhase μplates, provided in 1-, 2-, and 3-well formats for increased flexibility in crystallization trial setup. They are available as extra airtight NeXtal Evolution μplates, as 2-well MRC μplates, and as 3-well IntelliPlate low profile μplates. All plate formats are available precoated with either monoolein or monoolein plus 8% cholesterol. A choice of 2 sets of 96 screening solutions optimized for in meso experiments are also available. NeXtal CubicPhase I Suite contains buffered solutions with different added salts. NeXtal CubicPhase II Suite uses PEGs of differing molecular weight at set pH as precipitating agents.
Please note: The NeXtal CubicPhase System is phasing-out by December 31, 2016 and available only while stock lasts. Please contact your customer care department.
The NeXtal CubicPhase System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The innovative NeXtal CubicPhase crystallization system provides an optimized solution for the generation of high-quality membrane protein crystals (see figure "Crystal of bacterio-rhodopsin (BR)"). Only minute amounts of proteins (100 nl) are required compared to standard membrane protein crystallization setup.
Current methods for the setup of meso phase experiments include numerous manual steps. NeXtal CubicPhase crystallization products enable fully automatable, high-throughput membrane protein crystallization setup using a standard liquid-handling robot. Easy manual setup is also possible using a multichannel pipet. The NeXtal CubicPhase sytem utilizes the advantages of vapor diffusion crystallization together with those of in meso crystallization, which targets the phase transformation point from cubic to lamellar phase, as well as crystallization in the presence of excess water or sponge phase. The NeXtal CubicPhase system uses monoolein (Mo) alone (see figure "NeXtal CubicPhase 1w Mo μplate") or in combination with 8% cholesterol (C) as an additional experiment variable for greater flexibility. Addition of cholesterol allows reduction of the incubation temperature from 22°C to 18°C, which could be a critical experimental factor, especially for the most temperature-sensitive membrane proteins. NeXtal CubicPhase Suites have been tested and approved for compatibility with meso phase crystallization. Dedicated protocols for use with liquid-handling robots and multichannel pipets are included for different plate formats.
The NeXtal CubicPhase procedure is easy to perform (see figure "Workflow for crystallization experiments"). It does not require mixing of lipids and the protein solution prior to dispensing.
NeXtal CubicPhase μplates are compatible with the PX Scanner. The CubicPhase system is highly suited for:
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