Anti·His Antibody Selector Kit
For cost-effective comparison and determination of the optimal detection antibody for His-tagged proteins
Monoclonal mouse Anti·His Antibodies (IgG1) are used to detect recombinant proteins carrying His tags. Three antibodies are included (RGS·His, Penta·His, and Tetra·His), each 3 µg as BSA-free lyophilized powder, providing a 30 ml working solution. Each antibody detects a different His-tag epitope, with N-terminal, C-terminal, and internal His tags being detected by both Penta·His and Tetra·His antibodies, while RGS·His antibody is restricted to the N-terminus with a RGS-His tag. The antibodies have negligible cross-reactivity with crude E. coli, yeast, insect, or mammalian cell lysates.
The Anti·His Antibody Selector Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The sensitivity of the His Antibodies in western blots is up to 2 ng, and up to 0.5 ng in dot blots (see Figure "Sensitivity of QIAexpress Anti·His Antibodies"), when carrying out detection using a chromogenic substrate (sensitivity may vary with different proteins).
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee high purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. Tetra·His Antibody and Penta·His Antibody can both be used for detecting C-terminal, N-terminal, and internal His-tags. The RGS·His Antibody is restricted to His-tags at the N-terminus of proteins expressed with the RGS-His tag.
QIAexpress Detection Systems allow efficient detection of His-tagged proteins, and are ideal for many applications, including, colony, dot, and western blotting procedures (see figures "Detection of 6xHis-tagged thioredoxin in yeast cells", "Identification of positive expression clones by colony blotting", "Sensitivity of QIAexpress Anti·His Antibodies"), as well as screening for positive expression clones (see figure "Identification of positive expression clones by colony blotting"). QIAexpress Anti·His Antibodies and conjugates have many additional useful applications, including immunoprecipitation, ELISA, immunocytochemistry, immunohistochemistry (see figure "Immunohistochemistry with Penta·His Antibody"), protein localization and targeting studies, and LabMAP bead-based assays.
QIAexpress Detection Systems allow efficient detection of 6xHis-tagged proteins and are ideal for many applications, including:
fragment fix placeholder