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QIAprep Spin Miniprep Columns

For purification of up to 20 μg molecular biology grade plasmid DNA

  • Ready-to-use plasmid DNA in minutes
  • Reproducible yields of molecular biology grade plasmid DNA
  • Single protocol for high- and low-copy vectors

The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.

Cat No./ID: 27115
QIAprep 2.0 Spin Miniprep Columns (100 spin columns)
€93.40
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For high-purity plasmid minipreps
The QIAprep Spin Miniprep Columns is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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The QIAprep procedure.

1
Complete digestion with various restriction enzymes.
Restriction analysis of pBluescript DNA purified with the QIAprep Spin Miniprep Kit is shown. Digestion with the indicated enzymes (1–5 units) was carried out on 1 µg plasmid DNA. Markers: Lambda-HindIII.
2
Spin column handling options — B.
QIAvac 24 Plus.
Performance

The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep Spin Column handling options AB, and C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.

 
QIAprep Spin Miniprep Kit specifications
Format: Spin columns
Purification module: QIAprep spin columns
Throughput: 1–24 samples
Preparation time: 24 minipreps in 30 minutes
Equipment required: Microcentrifuge or vacuum manifold; fully automatable using the QIAcube
Lysate clearing: Centrifugation
Capacity of column reservoir: 800 µl
Minimum elution buffer volume: 50 µl
Culture volume for high-copy plasmids: 1–5 ml
Culture volume for low-copy plasmids/cosmids: 1–10 ml

Purified DNA can be used in restriction digestion (see figure "Complete digestion with various restriction enzymes"). 

Principle
QIAprep spin columns contain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenolchloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediately ready to use there is no need to precipitate, concentrate, or desalt.
Procedure

Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.

Applications

The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including:

PCR
Restriction digestion
Ligation and transformation
Sequencing 
Screening
Features
Specifications
Applications Fluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation etc.
Culture volume/starting material 1–10 ml culture volume
Elution volume 50 µl (minimal)
Plasmid type High-copy, low-copy, cosmid DNA
Processing Manual (vacuum or centrifugation)
Samples per run; throughput 1–24 samples per run
Technology Silica technology
Time per run or prep per run <30 minutes
Yield <20 µg
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