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QIAamp Media MDx Kit

For automated purification of DNA from liquid media
  • Purification from a variety of liquid transport media
  • Fully automated procedure with no centrifugation
  • Purification of viral and other DNA
  • High-quality DNA with efficient removal of contaminants

The QIAamp Media MDx Kit provides a fully automated procedure for purification of DNA from liquid media, such as cervical swab transport media. The fully automated process on the BioRobot MDx workstation, including bar code reading, load check, and complete process documentation, requires only 225 minutes for 96 samples, with no hands-on time.

Cat No./ID: 965752
QIAamp Media MDx Kit (12)
€4,819.00
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For 12 x 96 preps: 12 QIAamp 96 Plates, Buffers, Proteinase K, S-Blocks, Disposable Troughs, Racks with Elution Microtubes CL (0.4 ml), Carrier RNA, Top Elute Fluid, Caps, Tape Pad
The QIAamp Media MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
QIAamp Media MDx protocol.
1
Linear yields of high-quality nucleic acids.
Triplicate samples of SurePath liquid cytology medium were spiked with 103, 104, 105, and 106 Jurkat cells each. Total nucleic acids were purified using the QIAamp Media MDx Kit. A 9 µl aliquot from each eluate was used in a 25 µl real-time, quantitative PCR assay using the QuantiTect Probe PCR Kit with primers and probe specific for the β-actin gene.
2
Highly reproducible yields.
Forty-eight replicate samples of PreservCyt and SurePath liquid cytology media were spiked with 5 x 105 Jurkat cells each. Total nucleic acids were purified using the QIAamp Media MDx Kit. A 9 µl aliquot from each eluate was used in a 25 µl real-time, quantitative PCR assay using the QuantiTect Probe PCR Kit with primers and probe specific for the β-actin gene. [A] PreservCyt medium; [B] SurePath medium.
Performance
Purified nucleic acids are free of proteins, nucleases, and other impurities. Highly reproducible yields enable accurate results in downstream assays (see figure "Highly reproducible yields"). The high-quality nucleic acids are ready to use in downstream assays, such as real-time PCR (see figure "Linear yields of high-quality nucleic acids").
Principle

The QIAamp Media MDx Kit uses well-established technology for purification of nucleic acids from liquid media. The kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, for fully automated, simultaneous processing of up to ninety-six 265 µl samples on the BioRobot MDx.

Procedure
Liquid transport media can crosslink cells and form cellular aggregates that are difficult to lyse. Optimized buffers in the QIAamp Media MDx Kit efficiently lyse these samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane (see flowchart "Protocol"). Alcohol is added and lysates are loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities, including alcohols and phosphate, and pure, ready-to-use DNA is then eluted in a low-salt buffer. Patent-pending, computer-controlled vacuum technology ensures that volatile contaminants in liquid cytology media, such as alcohols, are efficiently removed.
Applications

The QIAamp Media MDx Kit can be used for purification of cellular, bacterial, and viral nucleic acids from a variety of sources, including:

Liquid cytology media with alcohol (e.g., PreservCyt and SurePath)
Phosphate-buffered liquid transport media (e.g., M4RT)
Urine
Dried blood spots, blood cards, and swabs
Features
Specifications
Applications PCR, real-time PCR
Elution volume 120 µl
For automated processing BioRobot MDx Workstation
Format 96-well plate
Main sample type Liquid media
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA
Sample amount 265 µl
Technology Silica technology
Time per run or per prep 200 minutes (96 samples)
Yield Varies
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