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QIAamp Investigator BioRobot Kit

For automated medium- to high-throughput purification of forensic DNA on the BioRobot Universal System

  • DNA purification from forensic and human identity samples
  • More efficient yields from trace casework samples
  • Higher signal-to-noise ratios
  • Rapid isolation of high-quality, ready-to-use DNA

The QIAamp Investigator BioRobot Kit provides automated DNA purification from up to 96 samples (such as filters, casework or crime-scene samples, and blood) or up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run. The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.

Cat No./ID: 965942
QIAamp Investigator BioRobot Kit (12)
€4,388.00
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For 12 x 96 DNA preps: QIAamp 96 Plates, S-Blocks, Buffers, Proteinase K, Elution Microtubes CL, Caps, Disposable Troughs
The QIAamp Investigator BioRobot Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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High-quality DNA from a wide range of sample types.
DNA was purified from the indicated sample types using the QIAamp Investigator BioRobot Kit (Investigator) on the BioRobot Universal System, or manually using the QIAamp DNA Micro Kit (Micro). Aliquots (5 μl, 10%) of each eluate were analyzed in triplicate by quantitative real-time PCR using the QuantiTect Probe PCR Kit with primers and probe specific for the β-actin gene. The mean threshold value (CT) and standard deviation are indicated for each sample.
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Superior DNA yields.
DNA (10 ng) was processed using QIAGEN automated purification procedures on the BioRobot Universal System (QIAamp), the BioRobot M48 (M48), the EZ1 Advanced (EZ1), or instruments from 2 other companies (Supplier I, Supplier P). Purified DNA was analyzed by quantitative real-time PCR using the QuantiTect Probe PCR Kit. The consistent low CT values for the QIAGEN purification procedures indicate higher yields and better recovery of the DNA.
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High-quality STR profiles.

DNA was purified from the indicated samples using the QIAamp Investigator BioRobot Kit in 8 separate runs on the BioRobot Universal System with a total of 48 purifications for each sample type. Each run included 46 or 47 negative samples in a checkerboard pattern with the positive samples. Full STR profiles were obtained from the indicated percentage of the positive samples. No cross-contamination was detected in any of the negative samples.

Performance

The QIAamp Investigator BioRobot Kit is suited for many applications, such as:

Purification of DNA from forensic casework and database samples
DNA purification from different trace samples (e.g., crime-scene)
Routine analysis of reference samples
Efficient yields from trace casework samples

The automated procedure is highly efficient, providing high yields of DNA (see figure "Superior DNA yields"). The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis (see figure "High-quality STR profiles"), with high signal-to-noise ratios. The kit provides high-quality DNA from 24 to 96 forensic samples in batches of 8 (see figure "High-quality DNA from a wide range of sample types").

Principle

The BioRobot Universal System together with the Application Pack, Investigator and the QIAamp Investigator Kit meet the demands of forensic laboratories with flexible, fully automated, standardized processing using proven QIAamp chemistry.

Procedure

The robust purification procedure provides high process safety and helps to prevent cross-contamination. For increased convenience and flexibility, there is the option of walkaway sample lysis and DNA purification or, if required, lysis can be performed manually followed by walkaway purification of DNA. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run.

Applications

The purification procedure of the QIAamp Investigator BioRobot Kit provides high-quality DNA from 24 to 96 forensic samples in batches of 8. Using the kit, the following samples can be processed together in a single run:

Dried blood
Surface swabs (e.g., blood, semen, saliva, and epithelial cells)
Stains on fabric (e.g., blood- or saliva-stained fabrics)
Cigarette butts and tobacco
Chewing gum and envelopes
Features
Specifications
Applications STR Typing, PCR, blotting
Elution volume 150 µl
For automated processing Biorobot Universal platform
Format 96-well plate
Main sample type Forensic casework and human identity samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 1 swab per well, up to 30 mg chewing gum, up to 40 mg cigarette butts, envelops, fabric or hair
Samples per run; throughput 24-96 samples per run
Technology Silica technology
Time per run or per prep <3 hours
Yield 1-2 µg DNA per swab; varying for casework samples

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Kit Handbooks (1)
For automated purification of DNA from forensic casework and human identity samples using the BioRobot Universal System
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