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PAXgene Blood DNA Tube (IVD)

For collection of whole blood for in vitro diagnostic (IVD) DNA testing
  • For In Vitro Diagnostic Use
  • Compatible with automated purification technologies
  • Validated for various collection and storage conditions
  • Unique blue cap and label identify tube for DNA testing
  • 2D barcode with tube ID, lot number and expiration date
The PAXgene Blood DNA Tube (IVD) is a blood collection tube that is robust to handling variability and easily integrated into standard automated workflows.
Cat No./ID: 761165
PAXgene Blood DNA Tube (IVD) (100)
100 PAXgene Blood DNA Tubes (IVD); available only via www.PreAnalytiX.com (cat. no. 761165)

The PAXgene Blood DNA Tube (IVD) is intended for in vitro diagnostic use.


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High DNA quality and yield from automated extraction on the QIAsymphony.
Samples collected in PAXgene Blood DNA Tubes (IVD) were processed on the QIAsymphony, which performs automated DNA purification based on magnetic bead extraction. Median DNA yield was 29.4 µg DNA/ml blood ranging from 16.9 to 51.7 at respective ends of the 95% tolerance interval. DNA purity is equally high, with an A260/A280 ratio of 1.7–1.9. Extraction was performed using QIAsymphony DSP DNA Kits.
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DNA quality and yield from automated extraction on the QIAcube.
Samples collected in PAXgene Blood DNA Tubes (IVD) were processed at 5 sites (4 external, 1 internal) on the QIAcube, which performs automated DNA extraction based on silica membrane technology. Median DNA yield was 22.5 µg DNA/ml blood, with 95% of samples ≥9.3 µg DNA/ml blood. DNA purity is high, with an A260/A280 ratio of 1.7–2.1. Extraction was performed using QIAamp DSP DNA Blood Mini Kits (200 µl blood sample input, 100 µl DNA eluate output).
Performance
The performance of the PAXgene Blood DNA Tube (IVD) has been validated under several processing temperatures and time intervals relevant to variation in blood collection techniques, transport methods and storage conditions. Validation information, along with data from exposure to high temperatures, long-term storage at –20°C and freeze-thaw tests are available under the Resources tab.

The table below summarizes DNA yields from whole blood collected in PAXgene Blood DNA Tubes (IVD) and purified using 2 different technologies. The yield of genomic DNA purified from blood samples collected in PAXgene Blood DNA Tubes (IVD) varies depending on the volume of blood processed, the white blood cell count of the specimen and the selected DNA isolation method.

Table 1. DNA yield from PAXgene Blood DNA Tubes (IVD) using 2 isolation technologies
 Extraction method  Number of samples  Input volume
(µl)
 Output volume
(µl)
 Lower 95% tolerance limit
(µg DNA/ml whole blood)
Median
(µg DNA/ml whole blood)
Mean
(µg DNA/ml whole blood)
 Magnetic beads (QIAsymphony)  581  200   200  18.2  28.9  30.2
 Silica membrane (QIAcube)  540  200   100  9.3  22.5  24.4


Principle
The PAXgene Blood DNA Tube (IVD) is a plastic, evacuated blood collection tube designed for in vitro diagnostic DNA testing. The tube includes features that minimize the risk of specimen misidentification: a distinctive blue rubber stopper in the Hemogard closure identifies the tube for DNA testing, and a 2D barcode on the label contains a unique tube identifier number, the tube lot number and the expiration date.
Procedure
Blood is collected into PAXgene Blood DNA Tubes (IVD) using standard phlebotomy techniques. The tube is designed to draw 2.5 ml of whole blood and this volume is easily identified by the fill indicator on the tube label. Venous whole blood can be stored in PAXgene Blood DNA Tubes (IVD) for up to 14 days at room temperature (18–25°C), 28 days at 2–8°C and for up to 52 weeks at –20°C.

For streamlined and standardized processing, PAXgene Blood DNA Tubes (IVD) are designed to integrate into automated workflows. For example, PAXgene Blood DNA Tubes (IVD) are compatible with DNA purification via magnetic beads in combination with QIAsymphony DSP DNA Kits (see figure DNA quality and yield from automated extraction on QIAsymphony). PAXgene Blood DNA Tubes (IVD) are also compatible with DNA purification via silica membrane in combination with QIAamp DSP DNA Blood Mini Kits (see figure DNA quality and yield from automated extraction on QIAcube).
Applications
PAXgene Blood DNA Tubes (IVD) can be used for molecular diagnostic methods requiring DNA.

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Brochures & Guides (1)
For collection of whole blood to be used for in vitro diagnostic (IVD) DNA testing
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Kit Handbooks (1)
For collection of whole blood to be used for in vitro diagnostic (IVD) DNA testing
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White Papers (1)
Performance of the PAXgene Blood DNA Tube (IVD) for collection, transportation, and storage of whole blood and the purification of DNA using the QIAsymphony instrument
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