text.skipToContent text.skipToNavigation

Gentra Puregene Tissue Kit

For purification of archive-quality DNA from tissues
  • Archive-quality DNA for long-term storage
  • Reproducible DNA purification
  • A complete solution for sample-to-storage purification
  • Convenient, scalable purification procedure

The Gentra Puregene Tissue Kit enables purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

Cat No./ID: 158667
Gentra Puregene Tissue Kit (4 g)
€274.00
Add To Cart
For 4 g tissue: RNase A Solution, Puregene Proteinase K, and Reagents
Cat No./ID: 158689
Gentra Puregene Tissue Kit (33 g)
€1,769.00
Add To Cart
For 33 g tissue: RNase A Solution, Puregene Proteinase K, and Reagents
The Gentra Puregene Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
1
High-molecular-weight DNA from tissue.
DNA was purified from 4 samples of muscle tissue using the Gentra Puregene Tissue Kit. Purified DNA (100 ng) was analyzed by agarose gel electrophoresis. M1: Uncut Lambda DNA; M2: Lambda HindIII marker.
Performance
Archive-quality DNA 

Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. The Gentra Puregene Tissue Kit removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb (see figure "High-molecular-weight DNA from tissue"). 

Principle
Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.
Procedure
The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA (see flowchart "Puregene DNA procedure"). No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.
Applications

DNA purified using the Gentra Puregene Tissue Kit is highly stable and suited for use in a wide range of applications, such as:

DNA archiving
PCR
SNP analysis
Southern blotting
Features
Specifications
Applications PCR, restriction digest, Southern analysis, SNP analysis
Elution volume 50-250 µl (varies)
Format Scalable
Main sample type Tissue samples, fixed and paraffin-embeded tissue
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 5-100 mg
Technology Modified salting-out precipitation method
Time per run or per prep 25 - 60 minutes (+ cell lysis + DNA rehydration)
Yield Varies

You are not authorized to download the resource

FAQs (22)
FAQ ID -1947
View
FAQ ID -1950
View
FAQ ID -1951
View
FAQ ID -1953
View
FAQ ID -1958
View
FAQ ID -1959
View
FAQ ID -1964
View
FAQ ID -1967
View
FAQ ID -1968
View
FAQ ID -1971
View
FAQ ID -1972
View
FAQ ID -1973
View
FAQ ID -1974
View
FAQ ID -2809
View
FAQ ID -2810
View
FAQ ID -2812
View
FAQ ID -2813
View
FAQ ID -618
View
FAQ ID -728
View
FAQ ID -754
View
FAQ ID -900
View
FAQ ID -908
View
Kit Handbooks (2)
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria
Show details
以下サンプルから長期保存に安定なゲノムDNAの精製: ヒト全血; 骨髄液 ; バフィーコート; 口腔細胞; 体液; 培養細胞; 組織; マウス尾; 酵母; バクテリア
Show details
Instrument Technical Documents (1)
Supplementary Protocols (18)
This protocol is designed for purification of DNA from 50 μl or 1 ml samples of clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from 2 ml samples of umbilical cord blood using the Gentra Puregene Tissue Kit or Gentra Puregene Blood Kit.
Show details
This protocol is designed for purification of DNA from 50 μl samples of dried human blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol provides information about scaling of reagents required for purification of DNA from 1 x 106 - 3 x 107 fixed cells using the Gentra Puregene Tissue Kit.
Show details
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-700 mg solid tissue using the Gentra Puregene Tissue Kit.
Show details
This protocol provides information about scaling of reagents required for purification of DNA from 0.5-20 mg paraffin-embedded tissue using the Gentra Puregene Tissue Kit.
Show details
This protocol is designed for purification of DNA from 1-3 ml samples of amniotic fluid containing 0.5-2 x 106 cells using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from 50 μl samples of CSF using the Gentra Puregene Tissue Kit.
Show details
This protocol is designed for purification of DNA from 1-2 ml samples of Chlamydomonas using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from 5-10 mg or 10-20 mg samples of fungal tissue using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from a fecal swab or 1-2 g samples of fecal material using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from 10-20 mg samples of fish tissue using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from 5-10 mg or 10-20 mg samples of solid tissue fixed in ethanol or formalin using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit.
Show details
This protocol is designed for purification of DNA from 5 hair roots using the Gentra Puregene Tissue Kit.
Show details
This protocol is designed for purification of DNA from 0.1-0.3 mg samples of bone fragments using the Gentra Puregene Tissue Kit.
Show details
This protocol is designed for purification of DNA from 5-20 mg samples of marine invertebrate tissue using the Gentra Puregene Tissue Kit.
Show details
This protocol is designed for purification of DNA from 40-90 mg (50-75 μl), 80-180 mg (100-150 μl), or 20 g samples of nematodes or nematode suspensions using the Gentra Puregene Tissue Kit.
Show details
This protocol is designed for purification of DNA from 1 ml samples of whole blood using the Gentra Puregene Tissue Kit.
Show details
References
0
fragment fix placeholder

Customers who bought these products also bought