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MagAttract DNA Blood Midi M48 Kit

For automated purification of genomic DNA from blood or buffy coat samples using the BioRobot M48

  • Rapid purification of up to 11 µg high-quality DNA
  • Flexible purification of 6–48 samples per run
  • Easy optimization of DNA concentration

The MagAttract DNA Blood Midi M48 Kit provides automated purification of genomic DNA on the BioRobot M48 using proven MagAttract magnetic particle technology. DNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure. Up to 48 samples can be processed per run in increments of 6 samples. Variable elution volumes enable easy optimization of DNA concentration.

Cat No./ID: 951356
MagAttract DNA Blood Midi M48 Kit (192)
€926.00
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MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 350 µl preps
The MagAttract DNA Blood Midi M48 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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MagAttract DNA Blood Midi M48 procedure.
1
Reproducible purification of high-quality genomic DNA.
High-quality genomic DNA purified from 48 blood samples using the BioRobot M48 and the MagAttract DNA Blood M48 Kit. Eluate (10 µl of each 200 µl) was analyzed by agarose gel electrophoresis.
2
Reproducible performance in sensitive PCR analysis.
Amplification of a single-copy gene (MECL-1) using template DNA purified from whole blood. Samples 1–6 and 43–48 are from the first and last sample prep plate positions on the BioRobot M48 worktable, respectively. All blood samples were obtained from the same donor. M: 100 bp DNA ladder (100 ng); +: positive control; –: negative control. A 5 µl aliquot (10%) of each PCR was loaded on the agarose gel.
3
High-quality genomic DNA from differently preserved whole-blood samples.
Amplification (34 cycles) of the MECL-1 single-copy gene (900 bp fragment) from a dilution series of DNA purified from 200 µl ACD-, heparin-, and EDTA-preserved whole blood samples. M: 100 bp DNA ladder (100 ng); C-: negative control; C+: positive control.
Performance
The MagAttract DNA Blood Midi M48 Kit provides fully automated purification of DNA (see figure "Reproducible purification of high-quality genomic DNA") and the purified DNA is free of PCR inhibitors (see figure  "Reproducible performance in sensitive PCR analysis"). The MagAttract DNA Blood Midi M48 Kit is suitable for use with ACD-, heparin-, and EDTA-preserved whole blood samples (see figure "High-quality genomic DNA from differently preserved whole-blood samples").
Principle

The MagAttract DNA Blood Midi M48 Kit is designed for fully automated DNA purification. Proven MagAttract magnetic particle technology in combination with the BioRobot M48 workstation provides high-quality DNA, suitable for direct use in sensitive downstream applications. Advantages of the system include scalable sample volumes and elution volumes (see table), giving the yield and concentration of high-quality DNA appropriate for the intended downstream application.

Sample and elution volumes for the MagAttract DNA Blood M48 procedure
SampleKit Protocol Amount of starting material (µl) Elution volume (µl)
Blood MagAttract DNA Blood Mini M48 Kit 200 µl Blood 100–200 50–400
Blood MagAttract DNA Blood Midi M48 Kit 350 µl Blood 250–350 100–400
Blood MagAttract DNA Blood Midi M48 Kit 700 μl Blood  500–700 200–400
Buffy coat enriched >9x MagAttract DNA Blood Midi M48 Kit 75 μl Buffy Coat 50–75 150–400
Buffy coat enriched ≤9x MagAttract DNA Blood Midi M48 Kit 150 μl Buffy Coat 100–150 150–400
Buffy coat with low leukocyte
concentration		 MagAttract DNA Blood Midi M48 Kit 300 μl Buffy Coat 200–300 150–400

Procedure
MagAttract technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. Optimized buffers lyse samples, and DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt (see flowchart "MagAttract DNA Blood Midi M48 procedure"). DNA bound to the magnetic particles is then efficiently washed. Two different wash buffers are used, followed by a rapid rinse with distilled water, which considerably improves the purity of the DNA. High-quality DNA is eluted in the water provided.
Applications

DNA purified using the MagAttract DNA Blood Midi M48 Kit can be used in a wide range of downstream applications, including:

PCR and quantitative, real-time PCR
SNP genotyping
Sequencing
Features
Specifications
Applications PCR, qPCR, real-time, genotyping, sequencing
Elution volume 100–400 µl
For automated processing BioRobot M48 Workstation
Format Sample tube (1.5 ml, 2 ml)
Main sample type Whole blood, buffy coat
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 250–350 µl
Technology Magnetic particles
Time per run or per prep 28 minutes – 3 hours
Yield 4.8–11.2 µg

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Kit Handbooks (1)
(EN) - MagAttract DNA Blood M48 Handbook (June 2012)
MagAttract DNA Blood Mini and Midi M48 Kit For DNA purification from human whole blood and buffy coat using the BioRobot M48 workstation
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