GeneRead DNAseq Library Quant Array
For qPCR-enabled quantification of NGS libraries before sequencing
The GeneRead DNAseq Library Quant Array provides a ready-to-use solution to quantify and qualify GeneRead DNAseq Targeted Panel V2 libraries before sequencing, enabling consistent and meaningful results with every NGS run.
The GeneRead DNAseq Library Quant Array uses real-time PCR to quantify NGS libraries by specifically quantifying DNA molecules with adaptors at both ends, which are the only amplifiable molecules during emulsion PCR (Ion Torrent platform) or bridge PCR (Illumina platform), and therefore provides highly accurate quantification of amplifiable library molecules. To determine the concentration of the experimental sample, simply create a standard curve using the values from the 5 sequential tenfold dilutions of DNA standard provided on the array plate, and find where the sample library falls (see figure, Principle of QIAGEN GeneRead DNAseq Library Quant System). The high sensitivity of real-time PCR allows quantification of libraries with very low concentrations, even below the detection threshold of conventional spectrophotometric methods.
The GeneRead DNAseq Library Quant Array also includes sets of QC primers to monitor the results of the target enrichment procedure. Each GeneRead DNAseq Gene Panel contains a set of endogenous controls, and the GeneRead DNAseq Library Quant Array provides predispensed primer assays to measure those controls for determination of the QC score of the prepared sample library. The QC score serves as a checkpoint to identify low-quality libraries before sequencing, preventing costly sequencing runs on samples that would not generate meaningful results. The GeneRead DNAseq Library Quant Array is optimized with GeneRead qPCR SYBR® Green Mastermixes to provide superior sensitivity and wide dynamic ranges.
The GeneRead DNAseq Library Quant Array is highly suited for library quantification and quality control in combination with GeneRead DNAseq Gene Panels.
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