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DNeasy PowerPlant Pro HTP 96 Kit

For high-throughput isolation of genomic DNA from plant and seed samples

  • High-quality DNA recovery from all plant types
  • High DNA yields even from plant material with high in polyphenolic compounds
  • High-throughput, 96-well format processes more samples per run
  • Inhibitor Removal Technology efficiently eliminates inhibitors for better downstream results
Process more plant samples with the high-throughput DNeasy PowerPlant Pro HTP 96 Kit. Recover high-quality DNA from tough sample types, including cotton leaves and seeds, strawberry leaves and pine needles. With this kit, time consuming DNA isolation procedures can be replaced.

Inhibitor Removal Technology eliminates inhibitors resulting in pure DNA that is ready to use in downstream applications including qPCR, Sanger sequencing and next-generation sequencing. Additionally, for samples high in polyphenolic compounds, such as pine and grape leaves, an optional step has been included in the kit with a unique Phenolic Separation Solution. This solution breaks the bonds between phenolic compound and DNA to prevent DNA loss and increase overall yield. DNeasy PowerPlant Pro HTP 96 Kit can be used in combination with other lysis methods and provides DNA ready for downstream applications.

DNeasy PowerPlant Pro HTP 96 Kit was formerly sold by MO BIO as PowerPlant Pro-HTP 96 Well DNA kit.
Cat No./ID: 13496-4
DNeasy PowerPlant Pro HTP 96 Kit (384)
€1,242.00
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For high-throughput isolation of genomic DNA from plant and seed samples
The DNeasy PowerPlant Pro HTP 96 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1.
Isolation of high quality genomic DNA from plant samples using the DNeasy PowerPlant Pro Kit. DNA isolated from grass (2 ul), strawberry leaf (15 ul), grape leaf (15 ul) and pine needles (1 ul) was examined on a 1% TAE agarose gel.
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Figure 2.

Successful amplification of DNA isolated using the DNeasy PowerPlant Pro Kit. qPCR was performed using primers for RuBisCo on 1 ng of DNA isolated from pine needles (A) and strawberry leaf (B). Undiluted DNA and samples diluted 1:10 both fell within the standard curve (purple lines), and the difference between the diluted and undiluted samples was approximately 3 cycles.

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Figure 3.

The DNeasy PowerPlant Pro Kit provides greater yields of high molecular weight genomic DNA. DNA was isolated from cotton seed using the DNeasy PowerPlant Pro Kit and plant DNA isolation kits from three other suppliers. All isolations were performed according to the manufacturer’s protocol. DNA (2% of elution volume) was examined on a 1% TAE agarose gel.

 

Performance

 
Features
Specifications
Bead size (not included with kit) Metal Beads are recommended for bead beating and can be purchased separately, catalog # 13117-500Metal Beads (not included with kit) are recommended for bead beating and can be purchased separately, cat. no. 13117-500.
Binding capacity Up to 20 µg per prep
Format Silica Spin Filter Plates
Processing Bead beating
Sample size Up to 50 mg
Sample types Processed plants and seeds
Storage temperature RNase A should be stored at 4°C The other kit reagents and components should be stored at room temperature (15-30°C)
Throughput 96-384 samples
Time per run or per prep 384 samples in less than one day
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