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DNeasy mericon 96 QIAcube HT Kit

For automated high-throughput isolation of DNA from raw or processed food samples
  • Purification of DNA from various types of food samples
  • No carryover of PCR inhibitors from complex food matrices
  • Accelerated CTAB procedure ensures efficient DNA extraction
  • Simple and reliable automated processing for cost and time savings
 
The DNeasy mericon 96 QIAcube HT Kit enables simple, automated purification of DNA from a variety of raw and processed foods on the QIAcube HT system. Using proven DNeasy silica-membrane technology in a convenient 96-well format, contaminants and inhibitors from complex food matrices are removed to yield high-quality DNA ready for downstream analysis. The kit forms part of the comprehensive QIAGEN food testing portfolio, which also features assays for pathogen and GMO DNA detection and ingredient authentication.
Cat No./ID: 69571
DNeasy mericon 96 QIAcube HT Kit (5)
€1,002.00
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For 480 preps: DNeasy 96 plates, QIAGEN Proteinase K, buffers
Cat No./ID: 950067
QIAcube HT Plasticware
€247.00
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Plasticware for 480 typical preps on QIAcube HT:
5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
The DNeasy mericon Food Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Efficient and rapid DNA extraction.
The DNeasy mericon 96 QIAcube HT Kit enables DNA extraction from food samples in radically shorter time compared to conventional CTAB protocols. Automated purification of 24–96 samples can be carried out on the QIAcube HT instrument in just a few hours with little hands-on time.

1
High yields of DNA and minimal inhibitor carryover.
DNA was purified from peanut butter and chocolate using the DNeasy mericon Food Kit and kits from 3 other suppliers. [A] Quantitative PCR for the trnL chloroplast tRNA gene showed substantially lower average CT values for DNA purified with the DNeasy mericon Food Kit. [B] To test for carryover of inhibitors, DNA was diluted 1:10 and the same segment was amplified. DNA purified with the DNeasy mericon Food Kit was inhibitor-free, indicated by a ΔCT within the expected range of 2.9–3.5. DNA purified with the other kits gave much lower ΔCT values, indicating strong inhibitor contamination.
2
Purification of DNA from complex food samples.
DNA was purified from orange juice, chocolate, and peanut butter using the QIAcube HT instrument and the DNeasy mericon 96 QIAcube HT Kit or manually using the DNeasy mericon Food Kit. Purified DNA was analyzed by qPCR of the trnL chloroplast tRNA gene using the QuantiFast SYBR® Green PCR Kit. The resulting CT values indicate similar performance from both purification methods. Diluting the input DNA 1:10 for qPCR analysis shows that all purified DNA was also free of PCR inhibitors.
Performance
The DNeasy mericon 96 QIAcube HT Kit is designed for rapid, automated purification of DNA from a variety of raw and processed food matrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples. DNeasy mericon purification kits provide a universally applicable extraction method that gives reliable results even when using strongly inhibitory, highly processed, fatty, acidic, high-, or low-DNA content foods (see figure High yields of DNA and minimal inhibitor carryover). The QIAcube HT platform, together with the dedicated DNeasy mericon 96 QIAcube HT Kit, enables automated mid- to high-throughput DNA purification from food sample types. Now it is possible to prepare the same high-quality DNA as from the manual DNeasy mericon Food Kit with a quick and simple automated procedure (see figure Purification of DNA from food samples).
Principle
The DNeasy mericon 96 QIAcube HT Kit enables automated purification of nucleic acids from up to 300 mg food sample material using the QIAcube HT instrument. The procedure yields high-quality DNA that performs well in PCR and other downstream applications.

The DNeasy mericon 96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
 
Kit specifications
Specification Description
Number of samples 24–96 samples (to be processed in increments of 8)
Sample input volume Up to 300 mg food material
Elution volume 200 μl
Duration 96 samples in approximately 73 minutes
24 samples in approximately 42 minutes
Procedure
The DNeasy mericon 96 QIAcube HT Kit uses a modified cetyltrimethylammonium bromide (CTAB) extraction procedure for rapid automated purification of DNA from food matrices on the QIAcube HT instrument (see figure Efficient and rapid automated DNA extraction). Homogenized food samples undergo a manual pretreatment step for efficient lysis using Food Lysis Buffer in combination with proteinase K. Chloroform extraction removes any CTAB-protein, CTAB-debris, or CTAB-polysaccharide complexes. The aqueous phase containing the DNA is transferred to a 96-well S-Block, and all subsequent  purification steps are automated on the QIAcube HT instrument. 

QIAcube HT adds Buffer PB to the aqueous phase and transfers the mixture to the DNeasy 96 plate. As vacuum is applied, nucleic acids are adsorbed onto the silica membrane while contaminants pass through. Washing removes any remaining contaminants and inhibitors, and the DNA is eluted in Buffer EB. The resulting purified DNA is ready for use in a downstream mericon real-time PCR assay.
Applications
The DNeasy mericon 96 QIAcube HT Kit yields high-quality DNA from a variety of raw and processed food matrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples. Purified DNA is suitable for a range of downstream applications:
  • Food safety and quality testing
  • Ingredient authentication
  • Detection of genetically modified organisms
  • Detection of potential allergens

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Brochures & Guides (2)
Protocol Files (4)
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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Q Protocol notes for purification of total DNA from food samples on the QIAcube HT using the DNeasy mericon 96 QIAcube HT Kit; use with the DNeasy mericon 96 QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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Procedure for installing Q Protocols for QIAcube HT Kits on the QIAcube HT and the QIAxtractor. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
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These Q Protocols and installation files can only be used with QIAcube HT Operating software version 4.17 and are not compatible with the new QIAcube HT Prep Manager software.

QIAcube HT Prep Manager Software already includes all standard protocols. Additional protocols and kit configurations can be easily installed. Check for available updates under the "Operating Software" section of the "Product Resources" tab.
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Instrument User Manuals (1)
Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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Quick-Start Protocols (2)
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
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Handbooks & Protocols (1)
Handbook for purification of total DNA from food samples on the QIAcube HT using the DNeasy mericon 96 QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.
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Kit Handbooks (1)
Handbook for purification of total DNA from food samples on the QIAcube HT using the DNeasy mericon 96 QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.
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