text.skipToContent text.skipToNavigation

QIAfilter Plasmid Kits

For fast purification of up to 10 mg transfection-grade plasmid or cosmid DNA

  • Lysate clearing without centrifugation
  • Reduced plasmid purification time
  • High yields of plasmid DNA
  • LyseBlue reagent for optimum lysis and maximum DNA yield

QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. Purified DNA is equivalent to that obtained by
2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

Cat No./ID: 12243
QIAfilter Plasmid Midi Kit (25)
€252.00
Add To Cart
25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges
Cat No./ID: 12245
QIAfilter Plasmid Midi Kit (100)
€913.00
Add To Cart
100 QIAGEN-tip 100, Reagents, Buffers, 100 QIAfilter Midi Cartridges
Cat No./ID: 12262
QIAfilter Plasmid Maxi Kit (10)
€228.00
Add To Cart
10 QIAGEN-tip 500, Reagents, Buffers, 10 QIAfilter Maxi Cartridges
Cat No./ID: 12263
QIAfilter Plasmid Maxi Kit (25)
€528.00
Add To Cart
25 QIAGEN-tip 500, Reagents, Buffers, 25 QIAfilter Maxi Cartridges
Cat No./ID: 12281
QIAfilter Plasmid Mega Kit (5)
€240.00
Add To Cart
5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Cat No./ID: 12291
QIAfilter Plasmid Giga Kit (5)
€463.00
Add To Cart
5 QIAGEN-tip 10000, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Cat No./ID: 19743
QIAfilter Midi Cartridges (25)
€139.00
Add To Cart
25 QIAfilter Midi Cartridges
Cat No./ID: 19763
QIAfilter Maxi Cartridges (25)
€231.00
Add To Cart
25 QIAfilter Maxi Cartridges
Cat No./ID: 19781
QIAfilter Mega-Giga Cartridges (5)
€121.00
Add To Cart
5 QIAfilter Mega-Giga Cartridges, Buffer FWB2

QIAfilter Plasmid Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Performance

The QIAfilter Plasmid Kits combines QIAfilter Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tips containing anion-exchange resin for efficient plasmid purification. Yields of up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), and 100 µg (midi) transfection-grade, high-copy plasmid DNA are achieved from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium).

For purification of low-copy plasmids and cosmids, QIAfilter Plasmid Mega Kits are a better choice than QIAfilter Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.

 

Principle

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

QIAfilter Mega-Giga Cartridges operate with house vacuum to efficiently clear even large volumes of bacterial lysate with minimal effort. QIAfilter Mega and Midi Cartridges have a syringe format and lysates are cleared rapidly by pushing the liquid through the filter.

The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.

Specifications

Features
QIAfilter Plasmid
Giga Kit
QIAfilter Plasmid
Mega Kit
QIAfilter Plasmid
Maxi Kit
QIAfilter Plasmid
Midi Kit
Applications Transfection, cloning, sequencing, etc. Transfection, cloning, sequencing, etc. Transfection, cloning, sequencing, etc. Transfection, cloning, sequencing, etc.
Culture volume/starting material 2.5 liters culture volume 500 ml – 2.5 liters culture volume 100–250 ml culture volume 25–50 ml culture volume
Plasmid type High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA
Processing Manual (filtration) Manual (filtration) Manual (filtration) Manual (filtration)
Sample per run 1 sample per run 1 sample per run 1 sample per run 1 sample per run
Technology Anion-exchange technology Anion-exchange technology Anion-exchange technology Anion-exchange technology
Time per run 280 min 190 min 120 min 110 min
Yield <10 mg <2.5 mg <500 µg <100 µg

Procedure

Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared rapidly by filtration. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.

Applications

DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection.

You are not authorized to download the resource

FAQs (31)
FAQ ID -1
View
FAQ ID -3
View
FAQ ID -1031
View
FAQ ID -1059
View
FAQ ID -1060
View
FAQ ID -1062
View
FAQ ID -310
View
FAQ ID -350
View
FAQ ID -352
View
FAQ ID -366
View
FAQ ID -411
View
FAQ ID -412
View
FAQ ID -413
View
FAQ ID -415
View
FAQ ID -416
View
FAQ ID -418
View
FAQ ID -572
View
FAQ ID -769
View
FAQ ID -798
View
FAQ ID -859
View
FAQ ID -861
View
FAQ ID -862
View
FAQ ID -864
View
FAQ ID -865
View
FAQ ID -198
View
FAQ ID -121
View
FAQ ID -417
View
FAQ ID -192
View
FAQ ID -203
View
FAQ ID -212
View
FAQ ID -1061
View
References
0
fragment fix placeholder

Customers who bought these products also bought