QIAamp 96 PowerFecal QIAcube HT Kit
For automated high-throughput purification of genomic DNA from fresh or frozen stool samples that are high in PCR inhibitors
The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.
The QIAamp 96 PowerFecal QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise on quality or reliability.
Consistently high DNA yields compared to similar kitsThe QIAamp 96 PowerFecal QIAcube HT Kit enables rapid purification of high-quality DNA (human and bacterial) from different stool samples. The fully automated purification procedure provides yields comparable to the manual procedure and with similar QIAGEN stool DNA purification kits (see figures Comparable human DNA recovery with all kits whether used manually or automated and Comparable bacterial DNA recovery with all kits whether used manually or automated). Automated purification of DNA from stool samples is quick, easy and reliable with QIAcube HT and the dedicated QIAamp 96 PowerFecal QIAcube HT Kit. Whether stool samples from 10 different animals were subjected to manual gDNA purification using the QIAamp Fast DNA Stool Mini Kit or automated using the dedicated instrument kit, the target could be successfully amplified in a real-time PCR assay, as indicated by comparable CT values (see figure Automated bacterial DNA purification from various animal stool samples gave comparable yields to the manual procedure).
Effective removal of inhibitorsStool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples. The QIAamp 96 PowerFecal QIAcube HT Kit efficiently eliminates PCR inhibitors from samples, enabling reliable detection of DNA, similar to other QIAGEN stool DNA purification solutions whether used manually or automated (see figure Efficient PCR inhibitor removal with all kits whether used manually or automated).
The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the proprietary Inhibitor Removal Technology (IRT) with two precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations of unbiased DNA. The fully automated purification procedure using the QIAamp 96 PowerFecal QIAcube HT Kit provides comparable yields of DNA, similar to other trusted QIAGEN stool DNA purification solutions whether used manually or automated (see figure Even for difficult-to-lyse gram-positive bacteria, all the kits yielded comparable DNA recovery whether used manually or automated).
Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.
The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.
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