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DirectPrep 96 Kits

For high-throughput, automated purification of sequencing-grade DNA

  • Cost-effective purification for high-throughput projects
  • Simple one-plate procedure
  • Automated and manual procedures available
  • Up to 96 samples in just 35 minutes
  • SBS plate standard for easy automation
DirectPrep 96 Kits provide 96-well purification plates suitable for vacuum or automated processing, yielding sequencing-grade plasmid DNA. These cost-effective Kits are highly suited for sequencing and screening projects. The lysis chemistry eliminates the need for a lysate clearing step.
The product must be used in conjunction with QIAvac Multiwell.
Cat No./ID: 27361
DirectPrep 96 MiniPrep Kit (4)
€634.00
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For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Buffers, Flat-Bottom Blocks and Lids, AirPore Tape Sheets, Tape Pads, Elution Microtubes RS, Caps
Cat No./ID: 962341
DirectPrep 96 BioRobot Kit (4)
€639.00
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For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Buffers, Flat-Bottom Blocks and Lids, 96-Well Microplates FB, AirPore Tape Sheets, Tape Pads

DirectPrep 96 Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


 


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DirectPrep 96 procedure.
The DirectPrep 96 method uses a modified lysis chemistry that eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using only one plate and a simple bind-wash-elute procedure. The DirectPrep 96 procedure is designed for use on the QIAvac Multiwell, the BioRobot 3000, or BioRobot 8000.
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High-quality DNA.
Typical fluorescent capillary sequencing result obtained with plasmid DNA purified using the DirectPrep 96 BioRobot Kit and the BioRobot 8000. Plasmid pUC19 was sequenced using BigDye terminator v. 3.1 chemistry in a cycle sequencing reaction on an ABI PRISM 3700 DNA Analyzer. The sequencing reaction was purified using the DyeEx 96 Kit.
Performance

The DirectPrep 96 Kits are designed for easy, cost-effective, high-throughput purification of high-copy-plasmid DNA. The system uses a simple one-plate procedure and a modified alkaline lysis chemistry that makes clearing of the lysate unnecessary. Up to 96 minipreps are prepared in 60–65 minutes on the BioRobot 3000 and BioRobot 8000 (both no longer available), or in 35 minutes using the QIAvac Multiwell. The purified DNA is suitable for use in standard downstream applications such as sequencing and automated fluorescent sequencing (see figure "High-quality DNA").

The DirectPrep 96 Kits provide DirectPrep 96 Plates, buffers, growth blocks, and other components required for preparation of 4 x 96 plasmid minipreps. The plates are standard format according to the Society for Biomolecular Screening.

Principle

The DirectPrep 96 method of high-throughput plasmid DNA isolation uses a novel lysis chemistry that eliminates the need for a lysate clearing step. Plasmid DNA purification is performed using only one plate and a simple bind-wash-elute procedure. This results in a protocol that is easier to perform than most existing methods. The DirectPrep 96 procedure is designed for use on the BioRobot 3000 or BioRobot 8000 workstations and can also be performed manually using the QIAvac Multiwell.

Specifications
Features DirectPrep 96 BioRobot Kit DirectPrep 96 MiniPrep Kit
Applications PCR, fluorescent sequencing, restriction digestion, etc. PCR, fluorescent sequencing, restriction digestion, etc.
Culture volume/starting material 1.25 ml culture volume 1.25 ml culture volume
Plasmid type High-copy High-copy
Processing Automated Manual (vacuum)
Samples per run; throughput 96 samples per run 96 samples per run
Technology Silica technology Silica technology
Time per run <65 minutes 35 min/plate
Yield <4 µg <4 µg

Procedure
All lysis steps are carried out in the same 96-well flat-bottom block used for growth of the bacteria. After alkaline lysis of the cells, the lysate is neutralized using an optimized buffer, which leads to a virtually clear lysate. Centrifugation or filtration of the lysate is not required. Isopropanol is added in order to optimize the DNA binding conditions, and lysates are transferred to the activated DirectPrep 96 plate where all subsequent steps take place (see flowchart "DirectPrep 96 procedure"). Plasmid DNA binds to the membrane, which is subsequently washed. After washing, the membrane is dried and the bound plasmid DNA is eluted. The DirectPrep 96 procedure gives typical yields of up to 4 µg DNA from 1.25 ml
E. coli culture grown in LB medium.
Applications

The DirectPrep 96 Kits provide ready-to-use plasmid DNA of a yield and quality especially suited for high-throughput applications, including sequencing, PCR, restriction digestion, and transformation.

Features
Specifications
Applications PCR, fluorescent sequencing, Restriction digestion etc.
Culture volume/starting material 1.25 ml culture volume
Plasmid type High-copy
Processing Manual (vacuum)
Samples per run; throughput 96 samples per run
Technology Silica technology
Time per run or prep per run 35 min/plate
Yield <4 µg

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References
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