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MagAttract PowerMicrobiome DNA/RNA Kit

For the automated, hands-free isolation of nucleic acids from stool and gut material
  • Optimized for automated, hands-free purification
  • High yields of DNA and RNA from tough stool, gut materials or biosolids
  • Improved downstream applications with Inhibitor Removal Technology
  • High-quality DNA and RNA isolation without inhibitors using innovative technology
Automate the isolation of nucleic acids from stool, gut material and biosolids with the MagAttract PowerMicrobiome DNA/RNA Kit. Up to 0.25 g of sample can be run through this protocol, isolating high-quality DNA and RNA. Inhibitor Removal Technology eliminates lipids, heme, polysaccharides and other PCR inhibitors from the sample and ClearMag magnetic particles bind pure RNA and DNA only, excluding further organic inhibitors from continuing downstream to other applications. The kit does not use chaotropic salts or ethanol as part of the binding and washing steps, eliminating a secondary source of enzyme-inhibiting contamination.

Protocols are available for the Thermo Scientific KingFisher, Eppendorf epMotion and Hamilton Star platforms.

The MagAttract PowerMicrobiome DNA/RNA EP and MagAttract PowerMicrobiome DNA/RNA KF were formerly sold by MO BIO as PowerMag Microbiome DNA/RNA EP (384) and PowerMag Microbiome DNA/RNA KF (384).
Cat No./ID: 27500-4-EP
MagAttract PowerMicrobiome DNA/RNA EP
€2,020.00
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For the automated, hands-free isolation of nucleic acids from stool and gut material
Cat No./ID: 27500-4-EP-BP
PowerBead Plates, Glass (4)
€307.00
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MagAttract PowerMicrobiome DNA/RNA EP Kit Components
Cat No./ID: 27600-4-KF
MagAttract PowerMicrobiome DNA/RNA KF
€1,991.00
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For the automated, hands-free isolation of nucleic acids from stool and gut material
The MagAttract PowerMicrobiome DNA/RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Figure 1.
High quality, intact RNA was isolated from samples (0.25 g) spiked with Enterococcus using the MagAttract PowerMicrobiome RNA/DNA EP on the epMotion 5075 TMX. No differences in yield or quality were observed between the four replicate samples. 
Performance


Features
Specifications
Bead size 0.1 mm glass0.1 mm glass
Binding capacity 500 µg per 20 µl of beads500 µg per 20 µl of beads
Format ClearMag TechnologyClearMag Technology
Processing Bead beatingBead beating
Sample size 0.25 g0.25 g
Sample types Processed soil, fecal, water, food, insects, swabs with PCR inhibitorsSoil, fecal, water, food, insects, swabs with PCR inhibitors
Storage temperature RNase A should be stored at 4°C
The other kit reagents and components should be stored at room temperature (15-30°C)Store at room temperature (15-30°C)
Throughput 96-well plate96-well plate
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