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FlexiTube siRNA Premix

For optimized, one-step siRNA transfection
  • Save time — just add to cells and incubate
  • Efficient transfection with reduced effort
  • Consistency across multiple experiments

FlexiTube siRNA Premix is an optimized mixture of siRNA and transfection reagent. Premixing of siRNA and reagent saves time by eliminating mixing and complex formation protocol steps. In addition, use of FlexiTube siRNA Premix removes the effort involved in estimating optimal siRNA-reagent ratios. FlexiTube siRNA Premix is available for human and mouse genes.

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You can search for the following terms:
  • Entrez Gene IDs (e.g., 835)
  • RefSeq IDs (e.g., NM_032983, NP_116765)
  • Gene symbols (e.g., CASP2)
  • Cat. no. (e.g., SI00299551, QT01342509)
  • Sanger ID or Accession (e.g., hsa-let-7b, MI0000063)
  • CpG loci identification numbers (CG#) (e.g., CG17753661)

Do not enter species information in the Search box. Use the drop-down list to select your species of interest.
When searching for miRNAs, do not omit the hyphens. Use hyphens or spaces (e.g., search for hsa-let-7b or hsa let 7b. Do not search for hsalet7b).

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Product Product no. Cat. no. List price:
FlexiTube siRNA Premix
Optimized siRNA–reagent mix; 0.75 nmol siRNA; provided in tubes
1027420 Varies
192,00 €
Enter your targets in search box above.
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The FlexiTube siRNA Premix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Rapid, efficient knockdown.
HepG2 cells were transfected with various amounts of FlexiTube siRNA Premix targeted against lamin A/C. All resulted in highly efficient lamin A/C knockdown with no need for optimization.
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Phenotype analysis after knockdown.
HeLa, MCF-7, and HepG2 cells were transfected with FlexiTube siRNA Premix containing AllStars Negative Control siRNA (Neg. control) or FlexiTube siRNA Premix containing AllStars Hs Cell Death Control siRNA (Cell death). After 72 hours, cells were examined by light microscopy. The cell death phenotype caused by AllStars Hs Cell Death Control siRNA was clearly visible.
Performance

The ratio of reagent to siRNA in FlexiTube siRNA Premix provides highly efficient transfection and gene knockdown (see figures "Rapid, efficient knockdown" and "Phenotype analysis after knockdown").

FlexiTube siRNA Premix contains FlexiTube siRNA, which is designed using highly innovative and sophisticated HP OnGuard siRNA Design to deliver high potency and specificity. siRNAs are designed using neural-network technology based on a large data set from RNAi experiments. siRNA design is then checked for homology to all other sequences of the genome using an up-to-date, nonredundant sequence database and a proprietary homology analysis tool that incorporates exciting design features.

Performance guarantee

FlexiTube siRNA Premixes come with a one-time-only replacement offer. If several FlexiTube siRNA Premixes for the same target gene are ordered and at least 2 of them do not provide ≥70% target gene knockdown, QIAGEN will provide 2 additional siRNAs free of charge, once only. You will be asked to provide supporting data, demonstrating that the siRNA failed to knock down the target gene by at least 70% at the mRNA level under appropriate transfection conditions. Supporting data should include transfection efficiency data, quantitative silencing data, and data showing ≥70% knockdown of a positive control. This offer is valid for up to 6 months after the date of delivery.

Principle
With FlexiTube siRNA Premix, siRNA for your gene of interest and highly efficient transfection reagent are already premixed and provided in a single tube. FlexiTube siRNA Premix includes a specialized buffer that increases the stability of siRNA-reagent complexes. The ratio of reagent to siRNA in FlexiTube siRNA Premix is preoptimized to provide the highest transfection efficiency and gene knockdown (see figures "Rapid, efficient knockdown" and "Phenotype analysis after knockdown").
Procedure

FlexiTube siRNA Premix is ready-to-use for cell transfection. Simply add to cells and incubate. This is a significant time and labor saving compared with a typical transfection experiment in which siRNA is diluted, mixed with transfection reagent, and then the mixture is incubated to allow siRNA-reagent complex formation prior to adding the complexes to the cells and incubating.

With FlexiTube siRNA Premix, RNAi experiments get off to a faster start, as there is no need to optimize siRNA to reagent ratio. Tedious optimization experiments involving multiple transfections are minimized or eliminated, as FlexiTube siRNA Premix provides siRNA and reagent premixed at an optimal ratio.

Multiple transfections can be performed from a single FlexiTube siRNA Premix. This reduces variability, enabling consistency across experiments and more reliable results.

Applications

FlexiTube siRNA Premix is ideal for functional genomics or pathway analysis with small numbers of gene targets.

Features
Specifications
Design HP OnGuard design
Format Tube
Guarantee/validation for validated siRNAs
Modification No
Number of transfections 50 standard transfections in 24-well format
Scale or yield 0.75 nmol
siRNA per target gene variable
Species Human, mouse
Target sequence provided Yes
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