Rotor-Gene Probe PCR Kit
For ultrafast, real-time PCR and two-step RT-PCR using sequence-specific probes
The Rotor-Gene Probe PCR Kit is designed for use with the Rotor-Gene Q and other Rotor-Gene cyclers, providing ultrafast, highly sensitive quantification of gDNA and cDNA targets with real-time PCR and two-step RT-PCR using sequence-specific probes. Outstanding performance is achieved through the combination of a specially optimized master mix and the unique Rotor-Gene cycler. For convenience, the master mix can be stored at 2–8°C.
The Rotor-Gene Probe PCR Kit is well suited for use in quantitative PCR analysis (see figures "Highly sensitive and reproducible detection", "High precision in real-time PCR"), and provides reliable detection over a wide range of templates (see table and figures "High precision in real-time PCR", "Reliable detection down to 1 cell", and "Wide dynamic range").
The Rotor-Gene Probe PCR Kit enables reliable real-time two-step RT-PCR quantification on the Rotor-Gene Q without the need for optimization of reaction and cycling conditions. The kit is designed for use with hydrolysis probes (e.g., TaqMan® probes). Highly specific amplification is assured through a balanced combination of K+ and NH4+ ions, which promote specific primer annealing, enabling high PCR specificity and sensitivity (see figure "Specific primer annealing"). Fast cycling without compromising performance is achieved using Q-Bond, a novel PCR additive that enables cycler run times of as low as 45 minutes (see figure "Fast primer annealing").
The ready-to-use Rotor-Gene Probe PCR Master Mix eliminates the need for optimization of reaction and cycling conditions. Just add template DNA, primers, and probe to the master mix and program the cycler. Instructions are provided in the detailed handbook supplied with the kit.
Hydrolysis probes (e.g., TaqMan® probes) can be used in combination with the Rotor-Gene Probe PCR Kit on the Rotor-Gene Q for fast and sensitive quantification — simply add the primer-probe mix and template to the master mix.
For optimal results in real-time two-step RT-PCR, we recommend synthesizing cDNA using the QuantiTect Reverse Transcription Kit. The kit provides fast cDNA synthesis in just 20 minutes with integrated removal of genomic DNA contamination.
The Rotor-Gene Probe PCR Kit is for fast, real-time PCR and two-step RT-PCR of gDNA or cDNA targets using sequence-specific probes on the Rotor-Gene Q. It is also compatible with Rotor-Gene 3000 and the Rotor-Gene 6000 cyclers.
For ultrafast, one-step qRT-PCR gene expression analysis of RNA targets using sequence-specific probes on Rotor-Gene cyclers, use the Rotor-Gene Probe RT-PCR Kit.
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