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Housekeeping Genes

For use in normalizing your relative gene expression profiling experiment

  • Simple procedure to quickly identify stable reference genes
  • Integrated online data analysis makes selection easy
  • Available in 96- and 384-well plates or 100 ring discs

RT2 Profiler Array Housekeeping Genes PCR Arrays are used to screen for potential normalization genes for use in real-time PCR analysis. Housekeeping genes encode proteins that are usually essential for the maintenance of cellular function and often remains constant under most experimental conditions. With this array, you can easily profile the expression of twelve (12) commonly used housekeeping genes in eight (8) samples using SYBR-green based qPCR.



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Housekeeping Genes
Product Product no. Cat. no. List price:
RT2 Profiler PCR Array
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330231 PABT-000Z varies
RT2 Profiler PCR Array
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330231 PADM-000Z varies
RT2 Profiler PCR Array
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330231 PAEC-000Z varies
RT2 Profiler PCR Array
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330231 PAFD-000Z varies
RT2 Profiler PCR Array
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330231 PAGG-000Z varies
RT2 Profiler PCR Array
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330231 PAHS-000Z varies
RT2 Profiler PCR Array
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330231 PAJJ-000Z varies
RT2 Profiler PCR Array
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330231 PAMM-000Z varies
RT2 Profiler PCR Array
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330231 PANZ-000Z varies
RT2 Profiler PCR Array
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330231 PAQQ-000Z varies
RT2 Profiler PCR Array
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330231 PARN-000Z varies
RT2 Profiler PCR Array
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330231 PASS-000Z varies
RT2 Profiler PCR Array
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330231 PAZF-000Z varies
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Housekeeping Genes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Performance
A guaranteed system

RT2 Profiler PCR Arrays are tested and optimized in combination with the RT2 SYBR® Green qPCR Mastermixes and the RT2 First Strand Kit. This testing means that RT2 Profiler PCR Array performance is guaranteed when all three of these components are used together.

Sensitivity

When using the RT2 First Strand Kit, start with as little as 1 ng or as much as 5 µg of total RNA per sample.

Reproducibility

The complete PCR array system demonstrates strong correlations across technical replicates, lots, and instruments with average correlation coefficients >0.99, ensuring reliable detection of differences in expression between biological samples.

Specificity

The PCR array system, with high-quality input RNA, yields single bands of the predicted size without primer-dimers or other secondary products, therefore providing the highly accurate real-time PCR results.

Uniform PCR amplification efficiency

Uniform PCR amplification efficiency is required for the PCR array technology to allow accurate comparisons of gene expression across all genes and all samples. The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees the high performance of every primer assay on PCR arrays.

Principle

RT2 Profiler PCR Arrays are reliable tools for analyzing the expression of a focused panel of genes. Each 96-well plate, 384-well plate or 100-well disc PCR array includes SYBR Green-optimized primer assays for a thoroughly researched panel of relevant, pathway- or disease-focused genes. RT2 Profiler PCR Arrays can also be customized to contain a panel of genes tailored to your specific research interests. The high-quality primer design and RT2 SYBR Green qPCR Mastermix formulation enable the PCR array to amplify 96 or 384 different gene-specific products simultaneously under uniform cycling conditions.

This combination provides the RT2 Profiler PCR Array with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory.

RT2 Profiler PCR Arrays are sensitive enough for use with RNA prepared from regular samples (0.1–5 µg RNA), FFPE samples, and small samples (1–100 ng RNA).

Procedure

Simply mix the cDNA template with the appropriate ready-to-use PCR mastermix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. RT2 Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.

Flexible layout and controls

RT2 Profiler PCR Arrays are available in 96-well plate, 384-well plate, and 100-well disc formats, and are used to monitor the expression of 84 or 370 genes related to a disease state or pathway, plus 5 housekeeping genes. Each RT2 Profiler PCR Array also includes control elements for:

Data normalization  
Genomic DNA contamination detection
RNA sample quality 
General PCR performance

Easy-to-use data analysis

Data can be analyzed using an easy-to-use Excel-based data analysis template or Web-based software. Data analysis is based on the ΔΔCT method with normalization of the raw data to either housekeeping genes.

Applications

RT2 PCR Profiler Arrays can be used in all areas of biological and medical research, including:

  • Cancer Inflammation and cytokine profiling
  • Stem cells
  • Neuroscience
  • Signal transduction pathways
  • Cell adhesion and cell migration
  • Biomarker screening and Validation

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