For automated purification of nucleic acids from up to 14 human, forensic, or molecular diagnostics samples
  • High-quality genomic DNA and RNA from a range of sample materials
  • Viral RNA/DNA and bacterial DNA from biomedical samples
  • Throughput of up to 14 samples per run
  • Effortless data management with full traceability
  • UV lamp to help minimize contamination risk

The EZ1 Advanced XL performs automated nucleic acid purification for a wide range of sample types relevant for molecular diagnostics, human identity testing, forensics, biomedical research, and gene expression analysis.

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Product Cat. no. List price:
EZ1 Advanced XL, System
Robotic workstation for automated purification of nucleic acids from up to 14 samples using EZ1 Kits: includes installation, training, 1-year warranty on parts and labor
9001874
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EZ1 Advanced XL, Priority Package
Robotic workstation for automated purification of nucleic acids from up to 14 samples using EZ1 Kits: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001875
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EZ1 Advanced XL, Priority Package Plus
Robotic workstation for automated purification of nucleic acids from up to 14 samples using EZ1 Kits: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001876
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Silicone Grease, BR M48 & BR EZ1
Silicone Grease, BR M48 & BR EZ1
9239334
17,30 €

EZ1 Advanced Instruments are intended to be used only in combination with QIAGEN kits indicated for use with EZ1 Advanced Instruments for the applications described in the kit handbooks.


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Simple protocol.|Flexibility and ease of use.|Standardization and traceability.|Safety and reliability.|
1. Insert the EZ1 Advanced XL Card with the desired protocol into the card slot and start the instrument. 2. Place samples and prefilled, sealed reagent cartridges into the instrument. Samples are processed automatically and purified nucleic acids are transferred to elution tubes. 3. Remove pure nucleic acids in 1.5 ml microcentrifuge tubes at a concentration suited to the downstream application.|A wide range of preprogrammed protocols, without the need for any manual data entry, are available on EZ1 Advanced XL Cards for purification of DNA, RNA, or viral nucleic acids. New cards may be purchased — increasing the range of purification protocols without any retraining or costly modifications to the instrument.|The EZ1 Advanced XL instrument sets new standards in data management. Barcode reading enables complete tracking of samples and reagents throughout the entire purification process. For increased process control and reliability, reagent identification and lot numbers are logged together with the expiration date, and a warning is issued if the expiration date has been exceeded. The run report is compatible with subsequent processing by a Laboratory Information Management System (LIMS).|The EZ1 Advanced XL has been designed to ensure high process safety, high performance, and user convenience. A sensor ensures that the instrument door remains closed during sample preparation, reducing contact of laboratory personnel with potentially infectious samples and protecting sample integrity. At the end of each run, an intelligent-design UV lamp decontaminates the inner surface of the instrument. If required, the instrument can be placed in a safety cabinet, providing increased user safety.|
Performance
Standardization and traceability

The EZ1 Advanced XL sets new standards in data management. Barcode reading enables complete tracking of samples and reagents throughout the entire purification process. Notes and assay identification information can also be easily entered. For increased process control and reliability, reagent identification and lot numbers are logged together with the expiration date, and a warning is issued if the expiration date has been exceeded. The run report, automatically generated at the end of each protocol, contains all relevant instrument information, including maintenance procedures and decontamination data. The instruments generate a pdf and a csv file after each run. Data can be easily transferred to a printer or computer within the network and the report file can be subsequently processed by a LIMS (Laboratory Information Management System).

Proven technology with improved performance

Proven EZ1 Kits, in combination with EZ1 Advanced XL Cards, ensure high-performance results in purification procedures. The unrivaled ease of use of the EZ1 Advanced XL enables fast and reproducible purification, without the need for specialized training or previous experience with laboratory automation. The extensive application range, including DNA purification from blood, tissues, and human identity and forensic samples, RNA purification from cells and tissues, and purification of viral nucleic acids from a range of sample types, delivers the flexibility that is essential for laboratories.

Safety and reliability

The EZ1 Advanced XL has been designed to ensure high process safety, high performance, and user convenience. A sensor ensures that the instrument door remains closed during sample preparation, reducing contact of laboratory personnel with potentially infectious samples and protecting sample integrity. At the end of each run, an intelligent-design UV lamp decontaminates the inner surface of the instrument. UV decontamination is highly effective in eliminating Gram-positive and Gram-negative bacteria (see tables). If required, the instrument can be placed in a safety cabinet, providing increased user safety.

E. coli colony numbers
Irradiation (min) Run

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30 1 0 0 0 0 0 0
30 2 0 0 0 1 0 0
30 3 10 0 0 0 0 3
30 Control 311 352 367 367 351 396
60 1 0 0 0 0 0 0
60 2 0 0 0 0 0 0
60 3 0 0 0 0 0 0
60 Control 348 307 326 326 396 385

Six replicates (20 μl each) of Escherichia coli cultures (17,750 CFU/ml) were irradiated using the integrated UV lamp of the EZ1 Advanced, followed by growth on plates in appropriate culture medium. Three independent irradiation runs were performed. Irradiation using the UV lamp was highly effective in eliminating bacteria.

 

 

 

 

 

 

 

S. haemolyticus colony numbers
Irradiation (min) Run

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6

 

 

 

 

 

 

 

30 1 12 10 17 2 6 4
30 2 0 0 0 0 0 0
30 3 10 0 2 11 0 3
30 Control 248 256 396 314 321 267
60 1 0 0 0 0 0 0
60 2 0 0 0 0 0 0
60 3 10 0 0 0 0 0
60 Control 264 305 368 292 347 388

Six replicates (20 μl each) of Staphylococcus haemolyticus cultures (15,700 CFU/ml) were irradiated using the integrated UV lamp of the EZ1 Advanced, followed by growth on plates in appropriate culture medium. Three independent irradiation runs were performed. Irradiation using the UV lamp was highly effective in eliminating bacteria.

 

 

 

 

 

 

 

Principle

Automated sample preparation enables efficient, standardized purification of nucleic acids. Manual sample handling is minimized, ensuring safety to the user and reliable processing of samples.

The EZ1 Advanced XL instrument uses proven EZ1 Kits for purification of DNA, RNA, and viral nucleic acids from a wide range of starting materials. Purified nucleic acids are ready for immediate use in downstream applications. The combination of an easy-to-use instrument, with error-free protocol selection and worktable setup, and prefilled, sealed reagent cartridges makes nucleic acid purification easy.

Procedure
Flexibility and ease of use

The self-contained EZ1 Advanced XL instrument ensures optimal ease of use and walkaway automation. A wide range of protocols is available for purification of DNA, RNA, or viral nucleic acids. Up to 14 samples can be processed per run in as little as 20 minutes. The instrument is operated via a keypad, and instrument information is displayed on the VFD (vacuum fluorescent display).

All processing steps are performed by the instruments — from piercing reagent cartridges to elution of pure nucleic acids. A separate computer is not required for operation, and EZ1 Kits include all reagents and accessories required to process your samples. EZ1 Kits provide prefilled, foil-sealed reagent cartridges that remain sealed until the instrument door is closed and the protocol run started, reducing the risk of contamination during setup.

If desired, up to 4 EZ1 Advanced XL instruments can be connected to an external computer, expanding throughput up to 56 samples per run. This enables you to easily adapt your laboratory setup to changing sample preparation requirements.

EZ1 Kits and EZ1 Advanced XL Cards for a wide range of applications

The EZ1 Advanced XL uses proven EZ1 Kits with preprogrammed protocols on EZ1 Advanced XL Cards. EZ1 Kits are available for a broad range of applications including work in human identity and forensics, biomedical research, and gene expression analysis (see table).

Combinations of EZ1 Advanced XL Cards and EZ1 Kits
Card Kit Sample
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 200 µl  Kit Blood and blood-derived samples
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 350 µl Kit Blood and blood-derived samples
EZ1 Advanced XL DNA Dried Blood Card EZ1 DNA Tissue Kit Dried blood
EZ1 Advanced XL DNA Buffy Coat Card EZ1 DNA Blood 350 µl Kit Buffy coat
EZ1 Advanced XL DNA Tissue Card EZ1 DNA Tissue Kit Tissues
EZ1 Advanced XL DNA Paraffin Section Card EZ1 DNA Tissue Kit Paraffin-embedded tissues
EZ1 Advanced XL DNA Buccal Swab Card EZ1 DNA Tissue Kit Buccal swabs
EZ1 Advanced XL DNA Investigator Card EZ1 DNA Investigator Kit Forensic and human identity samples
EZ1 Advanced XL DNA Bacteria Card EZ1 DNA Tissue Kit Human samples, swabs, biopsies, bacterial cultures
EZ1 Advanced XL Virus Card v2.0 EZ1 Virus Mini Kit v2.0 Viral DNA and RNA and bacterial DNA from serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swab samples
EZ1 Advanced XL RNA Card EZ1 RNA Universal Tissue Kit Any type of human or animal tissue
EZ1 Advanced XL DSP DNA Blood Card* EZ1 DSP DNA Blood Kit* Human whole blood
EZ1 Advanced XL DSP Virus Card* EZ1 DSP Virus Kit* Viral DNA and RNA and bacterial DNA from serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swab samples
* EZ1 Advanced XL DSP Cards and EZ1 DSP Kits are intended for in vitro diagnostic use in Europe, the United States, and Canada.
Applications

Nucleic acids purified using the EZ1 Advanced XL are ready for use in a wide range of downstream applications, including:

Oncology, forensics, and genetic identity testing
Genotyping
Infectious disease research, including bacterial genotyping
Gene expression analysis
Feature
Specifications
Features Touch LCD user interface, IC card with protocols/software, barcode reader, UV lamp
Instrument dimensions 510 x 510 x 570 mm (w x d x h)
Kits designed for this instrument QIAGEN EZ1 Kits
Pipetting range 50-1000 µl
Protocols/main application on this instrument Purification of DNA, mRNA, total RNA, and viral RNA and DNA
Samples per run; throughput 1–14 samples per run
Technical data of the instrument Weight 48 kg, 100–240 V AC, 50–60 Hz
Technology Magnetic-particle technology

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Validation Reports
1
Supplementary Protocols
3

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Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
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Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
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Handbooks & Protocols
2
This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for bone or teeth and is for the lysis and extraction of DNA from forensic samples of calcified tissue in less time than the standard EZ1 DNA Investigator protocol pretreatment.
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Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
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User-Developed Protocols
2
This protocol has been adapted by the North Louisiana Criminalistics Laboratory from the pretreatment for bone or teeth and is for the lysis and extraction of DNA from forensic samples of calcified tissue in less time than the standard EZ1 DNA Investigator protocol pretreatment.
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This protocol has been adapted by the San Diego Police Department (SDPD) Crime Laboratory from the standard QIAGEN pretreatment for casework samples, and is intended for the lysis and extraction of DNA from forensic samples of fired (casings) and unfired (cartridges) ammunition.
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