T315I RGQ PCR Kit
For detection of the T315I mutation in the ABL gene
The T315I RGQ PCR Kit enables detection of the T315I mutation in the ABL gene (exon 6) using real-time PCR on the Rotor-Gene Q 5plex HRM Instrument. The kit provides all necessary reagents, optimized for rapid and sensitive detection of a low percentage of mutant DNA in a background of wild-type genomic DNA. ARMS PCR technology combined with the Scorpions detection technology provides high sensitivity and specificity for the detection of specific mutations.
The T315I RGQ PCR Kit is intended for research use only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
The T315I RGQ PCR Kit is a qualitative ready-to-use kit for the detection of the T315I mutation in the ABL gene (exon 6) using polymerase chain reaction (PCR) on the Rotor-Gene Q 5plex HRM instrument.
The T315I RGQ PCR Kit utilizes two technologies — ARMS (Amplification Refractory Mutation System) and Scorpions — for detection of mutations in real-time PCR.
Allele- or mutation-specific amplification is achieved by ARMS. Taq DNA polymerase is effective at distinguishing between a match and a mismatch at the 3' end of a PCR primer. Specific mutated sequences are selectively amplified, even in samples where the majority of the sequences do not carry the mutation. When the primer is fully matched, the amplification proceeds with full efficiency. When the 3' base is mismatched, only low-level background amplification occurs.
Detection of amplification is performed using Scorpions. Scorpions are bifunctional molecules containing a PCR primer covalently linked to a probe. The fluorophore in this probe interacts with a quencher, also incorporated into the probe, that reduces fluorescence. When the probe binds to the amplicon during PCR, the fluorophore and quencher become separated. This leads to an increase in fluorescence from the reaction tube.
The T315I RGQ PCR Kit comprises a two-step procedure. In the first step, the control assay is performed to assess the total amplifiable T315I ABL DNA in a sample. In the second step, both the mutation and control assays are performed to determine the presence or absence of mutant DNA.
The T315I RGQ PCR Kit enables detection of the T315I mutation within the ABL gene (exon 6) in a background of genomic DNA.
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