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QuantiFERON Monitor (QFM) test

For detection of cell mediated immune response in immunosuppressed solid organ transplant patients
  • Assesses cell-mediated immune (CMI) response
  • Provides nonpathogen specific immune monitoring
  • Complements pathogen specific monitoring with QuantiFERON-CMV test
  • Aids in clinical management of solid organ transplant patients
QuantiFERON Monitor (QFM) test is an innovative in vitro diagnostic test that detects interferon γ (IFNγ) following incubation of heparinized whole blood with innate and adaptive immune response stimulants.
Cat No./ID: 0650-0101
QuantiFERON Monitor (QFM) Blood Collection Tubes (100)
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For drawing blood for use with the QFM assay, 100 each
Cat No./ID: 0650-0701
QFM LyoSpheres
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QuantiFERON Monitor LyoSpheres include: 10 vials of pelleted lyophilized stimulants (1 pellet per vial)

The QuantiFERON Monitor (QFM) test is intended for in vitro diagnostic use.


Performance
Use QFM to assess cell-mediated immune response in the immunosuppressed solid organ transplant population.
Principle
After incubation of heparinized whole blood with innate and adaptive immune response stimulants (Toll Like Receptor and T cell receptor agonists, respectively) QFM test detects cell-mediated immune function through the measurement of interferon gamma (IFNγ) in plasma by enzyme-linked immunosorbent assay.
Procedure
The QFM test is performed in stages:
  1. Collect whole blood into the QFM Blood Collection Tube.
  2. Add a QFM LyoSphere to the QFM Blood Collection Tube, and incubate the tube.
  3. After incubation, centrifuge the tube to isolate plasma.
  4. Measure IFNγ in the extracted plasma by ELISA and compare the responses to a range of expected values to characterize immune response of the subject.
Applications
QuantiFERON Monitor test may be used to detect cell-mediated immune response in immunosuppressed patients who have undergone solid organ transplantation. QFM test is complementary to QuantiFERON-CMV test in this population for posttransplant patient management.
Software
Software is available at www.QuantiFERON.com.

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