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QuantiFERON-CMV Kits

For monitoring of CMV-specific T cell immune responses
  • Predict risk of new and recurrent CMV disease (1)
  • Monitor transplant patient's level of anti-CMV immunity (2)
  • Determine both pre- and posttransplant CMV-risk stratification (1–4)
  • Guide therapeutic decision making (1)
QuantiFERON-CMV is an in vitro diagnostic test using a peptide cocktail simulating human CMV proteins to stimulate cells in heparinized whole blood (5). Detection of IFNγ responses by ELISA may indicate CMV immunity.
Cat No./ID: 0192-0301
QuantiFERON-CMV Single Patient Pack (SPP)
10 single test packs of QF-CMV blood collection tubes: each pack contains 3 specialized blood collection tubes (Nil tube, CMV Antigen tube, Mitogen tube)
Cat No./ID: 0350-0201
QuantiFERON-CMV ELISA Kit (2 x 96)
An enzyme-linked immunosorbent assay (ELISA) used to detect and measure IFNγ present in plasma. Includes: Microplate strips, Human IFNγ Standard, lyophilized; Green Diluent; Conjugate 100X Concentrate, lyophilized; Wash Buffer 20X Concentrate; Enzyme Substrate Solution; Enzyme Stopping Solution

QuantiFERON-CMV Kits are intended for in vitro diagnostic use in Europe.


References
  1. Kotton, C.N. et al. (2013) Transplantation 96, 333.
  2. Kumar, D. et al. (2009) Am. J. Transplant. 9, 1214.
  3. Walker, S. et al. (2007) Transplant. Infect. Dis. 9, 165.
  4. Westall, G.P. et al. (2008) Am. J. Transplant. 8, 1749.
  5. QuantiFERON-CMV Package Insert, December 2012, 1075110 Rev. 01.
  6. Fleming, T. et al. (2010) J. Med. Virol. 82, 433.
  7. Lisboa, L.F. et al. (2012) Am. J. Transplant. 93, 195.
  8. Weseslindtner, L. et al. (2012) Am. J. Transplant. 12, 2172.
  9. Manuel, O. et al. (2013) Clin. Infect. Dis. 56(6), 817.

0
Time to development of CMV disease in patients at the end of prophylaxis.
In a posttransplant setting, patients with a reactive QuantiFERON-CMV test remain free from CMV disease significantly more often, and for longer, than patients with a nonreactive QuantiFERON-CMV test after the cessation of antiviral prophylaxis. This indicates QuantiFERON-CMV may predict development of late-onset CMV in transplant recipients (2).
1
A theoretical model of QuantiFERON-CMV responses.
In a theoretical model of QuantiFERON-CMV responses in a posttransplant setting, during and/or following prophylaxis and immune suppression, robust immune responses are associated with a decreased risk of CMV disease occurrence. A robust immune response in the CMV Antigen tube may be indicative of immunity to CMV.
2
CE-IVD marked.
3
For in vitro diagnostic use.
Performance
QuantiFERON-CMV is an in vitro diagnostic test using a peptide cocktail simulating human CMV proteins to stimulate cells in heparinized whole blood. Detection of interferon-gamma (IFNγ) by enzyme-linked immunosorbent assay (ELISA) is used to identify in vitro responses to these peptide antigens that are associated with CMV infection.(1, 5) Loss of this immune function may be associated with development of CMV disease.
In a posttransplant setting, patients who have cellular immune response to CMV at the end of prophylaxis have a significantly lower risk of developing CMV disease than those who do not have a detectable immune response. This indicates that QuantiFERON-CMV may predict the development of late-onset CMV disease in transplant recipients (2, 4, 6–9)(see figure "Time to development of CMV disease in patients at the end of prophyplaxis").
Principle
QuantiFERON-CMV uses 3 specialized blood collection tubes (Nil, CMV Antigen, Mitogen). The CMV Antigen tubes are coated with peptides that simulate CD8+ specific epitopes of CMV proteins. Stimulation of a patient's T cells with CMV peptides results in the production of IFNγ in infected individuals. A robust IFNγ response in the CMV Antigen tube is indicative of immunity to CMV (see figure "A theorhetical model of QuantiFERON-CMV responses").
Procedure
Patient blood is collected by venipuncture directly into 3 blood collection tubes (Nil, CMV Antigen, Mitogen) and then incubated for 16 to 24 hours at 37°C. After incubation, plasma is harvested and the ELISA assay is performed to detect human IFNγ, using a standard curve and measurement of optical density. Calculations and test interpretation may be performed manually. QuantiFERON-CMV Analysis software is also available.
Applications
QuantiFERON-CMV is used to assess the level of CMV-specific immunity in patients at risk of developing CMV disease. QuantiFERON-CMV may assist clinicians to predict the risk of new and recurrent CMV disease, guide therapeutic decision-making, and improve patient health (1).

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QIAGEN Websites (1)
Learn about QuantiFERON-CMV products, read translations of package inserts for international customers, and more.
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