text.skipToContent text.skipToNavigation

artus M. tuberculosis PCR Kits CE

For quantitative detection of DNA of all members of the M. tuberculosis complex using real-time PCR
  • Compliance with EU IVD Directive 98/79/EC
  • High reliability using the internal control
  • Highly sensitive detection of as few as 0.23 copies/µl (RG kit)
  • Accurate quantitation using the 4 standards supplied
artus M. tuberculosis PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable detection and quantitation of DNA from all members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii) for in vitro diagnostic use.
Cat No./ID: 4555063
artus M. tuberculosis LC PCR Kit (24) CE
For 24 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4555065
artus M. tuberculosis LC PCR Kit (96) CE
For 96 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4555163
artus M. tuberculosis TM PCR Kit (24) CE
For 24 reactions on the ABI PRISM 7000, 7700, and 7900 HT SDS: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4555263
artus M. tuberculosis RG PCR Kit (24) CE
For 24 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4555265
artus M. tuberculosis RG PCR Kit (96) CE
For 96 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)

artus M. tuberculosis PCR Kits CE are intended for in vitro diagnostic use in Europe.


0
Reliable quantitation of the M. tuberculosis complex pathogen load.
Detection of the quantitation standards (M. tuberculosis RG/TM QS 1–4) on the Rotor-Gene using the artus M. tuberculosis RG PCR Kit. NTC: no template control.
1
M. tuberculosis.
2
CE-IVD-marked.
3
In vitro diagnostic medical device.
Performance

To ensure highest sensitivity, artus M. tuberculosis PCR Kits have been optimized to detect low numbers of mycobacterial DNA. The analytical sensitivity of the artus M. tuberculosis RG PCR Kit is 0.23 copies/µl in the PCR on the Rotor-Gene Q/6000 instrument.

Performance characteristics of artus M. tuberculosis RG PCR Kit
Kitartus M. tuberculosis RG PCR Kit
Validated sample types Human sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebral spinal fluid (CSF), stomach fluid, or peritoneal punction
Analytical sensitivity 0.23 copies/µl in the PCR
 Specificity  All members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii)

Principle

artus M. tuberculosis PCR Kits are based on the amplification and simultaneous detection of a specific region of the mycobacterial genome using real-time PCR. The kits provide high levels of specificity, sensitivity, and reproducibility.

Each artus M. tuberculosis PCR Kit provides 4 M. tuberculosis quantitation standards (see figure "Reliable quantitation of M. tuberculosis complex pathogen load"). Use of the standards enables accurate quantitation of pathogen load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical M. tuberculosis PCR is not reduced.

Specifications of artus M. tuberculosis RG PCR Kit
Kitartus M. tuberculosis RG PCR Kit
Validated sample types Human sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebral spinal fluid (CSF), stomach fluid, or peritoneal punction
Amplicon 159 bp region of the mycobacterial genome

Procedure

artus M. tuberculosis PCR Kits provide all necessary reagents optimized for reliable detection and quantitation of DNA of all members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii). Simply add template DNA to the ready-to-use PCR master mix and Mg solution, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For DNA purification, the QIAamp DNA Mini Kit is to be used to ensure legally validated performance.

Applications
artus M. tuberculosis PCR Kits enable rapid and sensitive detection of DNA of all members of the M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. microti, and M. pinnipedii) from sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebrospinal fluid (CSF), stomach fluid, and peritoneal punction with accurate quantification. Kits are available for use on the ABI PRISM 7000, 7700, and 7900HT SDS, on the LightCycler 1.1/1.2/1.5/2.0 Instruments, or on Rotor-Gene Q instruments.
Features
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp DNA Mini Kit
RUO/CE/ASR CE-IVD
Sample type Sputum, bronchoalveolar lavage (BAL), bronchial secretion, cerebrospinal fluid (CSF), stomach fluid, peritoneal punction
Thermal cycler ABI PRISM 7000, 7700, 7900HT SDS, LightCycler, Rotor-Gene 3000
What detected Mycobacterium tuberculosis DNA (M. tuberculosis, M. africanum, M. bovis...)

You are not authorized to download the resource

FAQs (71)
FAQ ID -1372
View
FAQ ID -1373
View
FAQ ID -1374
View
FAQ ID -1375
View
FAQ ID -1376
View
FAQ ID -1377
View
FAQ ID -1379
View
FAQ ID -1380
View
FAQ ID -1382
View
FAQ ID -1383
View
FAQ ID -1384
View
FAQ ID -1385
View
FAQ ID -1386
View
FAQ ID -1387
View
FAQ ID -1388
View
FAQ ID -1389
View
FAQ ID -1390
View
FAQ ID -1391
View
FAQ ID -1392
View
FAQ ID -1393
View
FAQ ID -1394
View
FAQ ID -1395
View
FAQ ID -1396
View
FAQ ID -1397
View
FAQ ID -1398
View
FAQ ID -1399
View
FAQ ID -1400
View
FAQ ID -1401
View
FAQ ID -1486
View
FAQ ID -1487
View
FAQ ID -1488
View
FAQ ID -1489
View
FAQ ID -1490
View
FAQ ID -1491
View
FAQ ID -1492
View
FAQ ID -1493
View
FAQ ID -1494
View
FAQ ID -1495
View
FAQ ID -1496
View
FAQ ID -1497
View
FAQ ID -1498
View
FAQ ID -1499
View
FAQ ID -1500
View
FAQ ID -1501
View
FAQ ID -1502
View
FAQ ID -1503
View
FAQ ID -1504
View
FAQ ID -1505
View
FAQ ID -1506
View
FAQ ID -1507
View
FAQ ID -1508
View
FAQ ID -1509
View
FAQ ID -1510
View
FAQ ID -1511
View
FAQ ID -1512
View
FAQ ID -1514
View
FAQ ID -1515
View
FAQ ID -1516
View
FAQ ID -1517
View
FAQ ID -1518
View
FAQ ID -1520
View
FAQ ID -1521
View
FAQ ID -1522
View
FAQ ID -1523
View
FAQ ID -1524
View
FAQ ID -1525
View
FAQ ID -1527
View
FAQ ID -1529
View
FAQ ID -1534
View
FAQ ID -1692
View
FAQ ID -1528
View
fragment fix placeholder

Customers who bought these products also bought