artus C. trachomatis TM PCR Kit CE
For quantitative detection of Chlamydia trachomatis DNA using real-time PCR
The artus C. trachomatis PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable C. trachomatis DNA detection and quantitation for in vitro diagnostic use.
The artus C. trachomatis TM PCR Kit CE is intended for in vitro diagnostic use in Europe.
To ensure highest sensitivity, the artus C. trachomatis TM PCR Kit has been optimized to detect low numbers of C. trachomatis DNA. The analytical sensitivity of the artus C. trachomatis TM PCR Kit is 0.2 copies/µl in the PCR on the ABI PRISM 7700 and 7900HT SDS.
The artus C. trachomatis TM PCR Kit is based on the amplification and simultaneous detection of a specific region of the C. trachomatis genome using real-time PCR. The kit provides high levels of specificity, sensitivity, and reproducibility.
Each artus C. trachomatis TM PCR Kit provides 4 C. trachomatis quantitation standards (see figure "Reliable quantitation of C. trachomatis load"). Use of the standards enables accurate quantitation of pathogen load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical C. trachomatis PCR is not reduced.
The artus C. trachomatis TM PCR Kit provides all necessary reagents optimized for reliable C. trachomatis DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
For DNA purification, the QIAamp Viral RNA Mini Kit or QIAamp DNA Mini Kit is to be used.
The artus C. trachomatis PCR Kits enables rapid and sensitive detection of C. trachomatis DNA from urine, swabs, and semen with accurate quantification. The kit is available for use on the ABI PRISM 7000, 7700, and 7900HT SDS.
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