text.skipToContent text.skipToNavigation

mericon GMO Screen bar Kit

For real-time PCR detection of the bar gene in food or animal feed
  • Highly sensitive and specific detection of GMOs with the bar gene
  • Streamlined detection protocol
  • Dedicated mericon DNA isolation kit available
  • PCR run time of 82 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs
The mericon GMO Screen bar Kit is designed for the target-specific detection of the bar gene in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for pathogen detection and ingredient authentication. The mericon GMO Screen bar Kit performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
Cat No./ID: 291063
mericon GMO Screen bar Kit (24)
€263.00
Add To Cart
For 24 reactions: PCR Assay Screen bar, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution
Cat No./ID: 291065
mericon GMO Screen bar Kit (96)
€981.00
Add To Cart
For 96 reactions: PCR Assay Screen bar, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution
The mericon GMO Screen bar Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The mericon Screen bar Kit is designed for the detection of the bar gene in food and animal feed. The mericon Screen bar Kit can detect as little as 10 copies of target DNA in a reaction and exhibits high specificity for the bar gene. No cross-reactivity was observed with other genetic constructs or with plant or animal species not containing this genetic construct using 50 ng of tested DNA (see the table "Results of mericon GMO Screen bar Kit cross-reactivity study").

Results of mericon GMO Screen bar Kit cross-reactivity study

 Species, GMO, or
 genetic construct
 Result  Species, GMO or 
 genetic construct
 Result
 Bt176 corn  +  EH92-527 potato  –
 MON810 corn  –  H7-1 sugar beet  –
 Bt11 corn  –  Soy  –
 MIR604 corn  –  Barley  –
 3272 corn  –  Potato  –
 NK603 corn  –  Rice  –
 TC1507 corn  –  Wheat  –
 59122 corn  –  Rye  –
 98140 corn  –  Cattle  –
 Roundup Ready soy  –  Pig  –
 305423 soy  –  Sheep  –
 356043 soy  –  Goat  –

Principle

The mericon GMO Screen bar Kit is designed for the qualitative detection of genetically modified organisms (GMOs) containing the bar gene. The gene, which originates from Streptomyces hygroscopicus, transcripts phosphinotricin N-Acetyl-transferase and confers resistance to the active compound phosphinotricin of herbicides.

Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets either a genetic construct that is commonly used in GMOs or a DNA sequence that is specific for a particular GMO. These targets were verified bioinformatically and experimentally. 

Summary of possible PCR outcomes
Internal control amplifiedTested DNA amplified Meaning
            +            +  Sample is positive for the target DNA
            +            –  Sample is negative for the target DNA
            –            –  PCR failed due to inhibition
Procedure

DNA is extracted from a sample of food or animal feed using a dedicated sample preparation kit. QIAGEN's DNeasy mericon Food Kit is suitable for a wide range of food matrices, including those with high inhibitor contents.

The isolated, pure DNA is assayed using a streamlined real-time PCR protocol that returns results in 82 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications
The mericon GMO Screen bar Kit is for real-time PCR-based detection of GMOs with the bar gene in food, animal feed, and pharmaceutical products.
Features
Specifications
Quantitative/qualitative Qualitative
Recommended sample prep DNeasy mericon Food Kit or DNeasy Plant Kits
RUO/CE/ASR RUO
Sample type Food
Thermal cycler Rotor-Gene Q and other cyclers
What detected GMO DNA
fragment fix placeholder

Customers who bought these products also bought