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mericon Campylobacter triple Kit

For real-time PCR detection of Campylobacter jejuni, C. lari, and C. coli in food or animal feed
  • Highly sensitive and specific detection of Campylobacter DNA
  • Streamlined detection protocol
  • Dedicated mericon bacterial DNA isolation kits available
  • PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs
The mericon Campylobacter triple Kit is designed for the target-specific detection of Campylobacter jejuni, C. coli, and C. lari DNA in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol and forms part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. The mericon Campylobacter triple assay performs optimally on the Rotor-Gene Q, but has also been validated for block thermal cyclers.
Cat No./ID: 290043
mericon Campylobacter triple Kit (24)
€277.00
Add To Cart
For 24 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
Cat No./ID: 290045
mericon Campylobacter triple Kit (96)
€1,032.00
Add To Cart
For 96 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
The mericon Campylobacter triple Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The mericon Campylobacter triple Kit is for the specific detection of Campylobacter jejuni, C. coli, and C. lari in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Campylobacter jejuni, C. coli, orC. lari  DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter triple Kit cross-reactivity study").

Results of mericon Campylobacter triple Kit cross-reactivity study

 Pathogen  Result  Pathogen  Result
 Campylobacter jejuni  +  Campylobacter coli  +
 Campylobacter lari  +  Campylobacter upsaliensis  –
 Salmonella abony  –  Staphylococcus aureus  –
 Clostridium perfringens  –  Escherichia coli  –
 Legionella erythra  –  Yersinia enterocolitica  –
 Legionella pneumophila  –  Listeria monocytogenes  –
 Bacillus cereus  –  Cronobacter sakazakii  –
 Shigella flexneri  –  

Principle

The mericon Campylobacter triple Kit is designed for the qualitative detection of Campylobacter jejuni, C. coli, and C. lari in food and animal feed samples after enrichment. Campylobacter species are the second most frequent causative organisms of food-related diarrhea after Salmonella spp. The most common transmission routes are contaminated poultry products, ground meat, raw milk, or water.

Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibition. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Summary of possible PCR outcomes
Internal control amplifiedTested DNA amplified Meaning
+ + Sample is positive for the target DNA
+ Sample is negative for the target DNA
PCR failed due to inhibition
Procedure

DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.

The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications
The mericon Campylobacter triple Kit is for real-time PCR-based detection of Campylobacter jejuni, C. coli, and C. lari in food, animal feed, and pharmaceutical products.
Features
Specifications
Quantitative/qualitative Qualitative
Recommended sample prep mericon DNA Bacteria Kit (Gram-negative bacteria) or mericon DNA Bacteria Plus Kit (Gram-positive bacteria)
RUO/CE/ASR RUO
Sample type Bacterial preculture from food
Thermal cycler Rotor-Gene Q and other cyclers
What detected DNA of respective pathogen
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