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virotype PEDV/TGEV RT-PCR Rgt

For rapid identification of PEDV and TGEV RNA
  • Simultaneous identification of RNA from PEDV and TGEV
  • Ready-to-use master mix with internal control to minimize false negatives
  • Identification of substantially relevant strains
  • Reliable results within 2 hours

The virotype PEDV/TGEV RT-PCR Rgt contains all of the necessary reagents for the identification of PEDV and TGEV RNA, as well as a positive control. With this reagent, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination.

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Cat No./ID: 283615
virotype PEDV/TGEV RT-PCR Rgt (96)
For 96 reactions: Master Mix, Positive Control

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.


Principle
This is a real-time RT-PCR reagent for the identification of RNA from Porcine Epidemic Diarrhea Virus (PEDV) and Transmissible Gastroenteritis Virus (TGEV). RT-PCR is based on the amplification of specific regions of the pathogen genome. It is a sensitive and specific tool for pathogen identification.

Real-time RT-PCR involves the reverse transcription of viral RNA and subsequent amplification of the cDNA by PCR. The amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run in real time allows the the accumulating product to be identified, without the need to re-open the reaction tubes.
Procedure
Prior to real-time RT-PCR, viral RNA must be extracted from the starting material. QIAGEN offers a range of products for RNA extraction from animal samples.
Applications
The virotype PEDV/TGEV RT-PCR Rgt is designed to identify RNA from PEDV and TGEV in a range of biological samples using the polymerase chain reaction (PCR).

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