bactotype MAP PCR Kit
For sensitive and highly-specific detection of DNA from Mycobacterium avium subsp. paratuberculosis
MAP is the cause of paratuberculosis (Johne's disease), a chronic inflammatory intestinal disease of ruminants, which occurs worldwide. The bactotype MAP PCR Kit is a highly sensitive and specific solution for the detection of DNA from Mycobacterium avium subsp. paratuberculosis (MAP) in fecal samples and tissue samples (e.g., small intestine, mesenterial lymph nodes) from ruminants and culture.
The bactotype MAP PCR Kit is for veterinary use only. In Germany: Licensed in accordance with §17c German Animal Disease Act (pending).
Polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterwards. The bactotype MAP PCR Kit contains all of the necessary reagents for the detection of MAP DNA, including a positive control, a negative control and an internal control.
The internal control DNA permits tests for successful purification and amplification by adding to the DNA purification procedure. The kit uses two specific primer/probe combinations: one for MAP DNA yielding FAM fluorescence and one for the internal control yielding MAX fluorescence. The Positive Control contains MAP DNA and serves to prove the functionality of the pathogen assay, for example, the correct setup of the reaction mix.
The kit contains a ready-to-use mix with all of the necessary reagents for the detection of DNA from Mycobacterium avium subsp. paratuberculosis (MAP) and the required positive and negative controls.
DNA is extracted from the starting material (e.g., feces) using a suitable sample preparation kit. Please refer to the supplementary protocols associated with the QIAamp cador Pathogen Mini Kit and/or QIAamp DNA Stool Mini Kit. The extracted DNA is used for the PCR, which is run on a thermal cycler.
The bactotype MAP PCR Kit is for detection of DNA from Mycobacterium avium subsp. paratuberculosis (MAP) in samples from ruminants and culture slants.
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