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QIAsymphony RGQ

Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs

  • Unprecedented flexibility
  • Workflow optimization and utility
  • Maximized ease of use and convenience
  • Regulated assays and laboratory-developed tests
  • Designed for largest range of applications

QIAGEN's premier sample-to-result workflow solution comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and a broad range of ready-to-use assays on the Rotor-Gene Q real-time PCR cycler. QIAGEN continues to expand the QIAsymphony series with new features, assay menus, and workflows.

Explore the virtual demo to learn more about QIAsymphony RGQ.
Cat No./ID: 9001850
QIAsymphony RGQ, System
QIAsymphony SP, QIAsymphony AS, Rotor-Gene Q 5plex HRM; includes required accessories and consumables, installation, and training; includes 1-year warranty on parts and labor
Cat No./ID: 9020246
QIAsymphony Cabinet SP/AS
Accessory for correct positioning of the QIAsymphony SP/AS instruments

The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN kits indicated for use with the QIAsymphony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN kits, it is the users responsibility to validate the performance of such product combination for any particular application. The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is intended for the applications described in the respective QIAGEN kits handbooks. If the Rotor-Gene Q instrument is used with kits other than QIAGEN kits, it is the users responsibility to validate the performance of such product combination for any particular application.



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QIAsymphony workflows.

There are 2 modes of system operation: integrated or independent.

1
Highly sensitive detection of CMV DNA.

Probit analysis: CMV (QIAsymphony RGQ system). Analytical sensitivity of the artus CMV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.

2
Highly sensitive detection of EBV DNA.

Probit analysis: EBV (QIAsymphony RGQ system). Analytical sensitivity of the artus EBV QS-RGQ Kit with the QIAsymphony RGQ system using EDTA plasma.

3
Highly sensitive detection of HBV DNA.

Probit analysis: HBV (QIAsymphony RGQ system). Analytical sensitivity of the artus HBV QS-RGQ Kit with the QIAsymphony RGQ system.

4
Highly sensitive detection of HCV RNA.

Probit analysis: HCV (QIAsymphony RGQ system). Analytical sensitivity of the artus HCV QS-RGQ Kit with the QIAsymphony RGQ system.

5
Highly sensitive detection of HIV-1 RNA

Probit analysis: HI Virus-1 (QIAsymphony RGQ system). Analytical sensitivity of the artus HI Virus-1 QS-RGQ Kit with the QIAsymphony RGQ system.

Performance
The QIAsymphony RGQ, in combination with artus QS-RGQ Kits, enables highly sensitive and specific detection of:
CMV DNA (see figure "Highly sensitive detection of CMV DNA")
EBV DNA (see figure "Highly sensitive detection of EBV DNA")
HBV DNA (see figure "Highly sensitive detection of HBV DNA")
HIV-1 RNA (see figure "Highly sensitive detection of HIV-1 RNA")
HCV RNA (see figure "Highly sensitive detection of HCV RNA")
Principle
QIAsymphony RGQ can save you time by processing up to 96 samples, from sample to result, in one integrated run. A dedicated range of kits enable open or closed systems for molecular testing.

QIAsymphony RGQ is the integrated system to maximize your efficiency and optimize your workflows, from sample to result. QIAsymphony RGQ synergizes the automated sample preparation and assay setup on the QIAsymphony SP/AS with manual transfer to the trusted Rotor-Gene Q for detection. The QIAsymphony SP automates sample preparation from a wide range of sample types. The QIAsymphony AS delivers highly reliable and reproducible PCR assay setup and is highly suited for laboratories that perform complex PCR-based molecular testing on a day-to-day basis. The QIAsymphony AS enables setup of multiple assays per run or sample and supports artus QS-RGQ products, for a complete CE-IVD compliant workflow, as well as in-house assays. The Rotor-Gene Q enables sensitive, precise real-time PCR analysis with CE-IVD-marked artus QS-RGQ Kits or in-house assays.

Kits for complete CE-IVD compliant workflow on the QIAsymphony RGQ
Kits Instrument Method
QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
artus QS-RGQ Kits QIAsymphony AS Real-time PCR (multiplex; sample and internal control)
Kits for in-house tests on the QIAsymphony RGQ
Application Kits Instrument Method
Purification QIAsymphony DSP AXpH DNA Kit QIAsymphony SP Purification of DNA from liquid-based cytology media
QIAsymphony DSP DNA Kits QIAsymphony SP Purification of DNA from human whole blood, buffy coat, tissues, FFPE tissues, cultured cells and bacterial cultures, as well as viral DNA from human whole blood
QIAsymphony DSP Virus/Pathogen Kits QIAsymphony SP Purification of viral nucleic acids or bacterial DNA from a broad range of sample types
QIAsymphony Certal Kits QIAsymphony SP Purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations
Assay setup — pathogen detection QuantiFast Pathogen +IC Kits QIAsymphony AS Multiplex real-time PCR and RT-PCR using sequence-specific probes
QuantiTect Virus Kits QIAsymphony AS Highly sensitive detection of multiple viral DNA and RNA targets in the same tube
QIAGEN OneStep RT-PCR Kit QIAsymphony AS Sensitive RT-PCR, with simple setup, using any RNA template
Assay setup — gene expression Rotor-Gene SYBR® Green Kits QIAsymphony AS Ultrafast, precise real-time PCR and RT-PCR using SYBR® Green I
Rotor-Gene Probe Kits QIAsymphony AS Ultrafast, precise real-time PCR and RT-PCR using sequence-specific probes
Rotor-Gene Multiplex Kits QIAsymphony AS Ultrafast, precise multiplex real-time PCR and RT-PCR using sequence-specific probes
Assay setup — residual DNA detection Certal Detection Kits QIAsymphony AS Duplex real-time PCR detection of resDNA target and a generic internal control
Procedure

The QIAsymphony RGQ workflow starts with purification of nucleic acids on the QIAsymphony SP instrument. The eluates, containing purified nucleic acids from the sample preparation procedure, are transferred to the QIAsymphony AS module, which performs assay setup. The assays are then manually transferred to the Rotor-Gene Q for PCR and data analysis.

To suit individual workflow requirements, there are 2 modes of system operation: integrated or independent. In the integrated mode, samples processed on the QIAsymphony SP are transferred automatically to the QIAsymphony AS, reducing manual handling steps and documentation. For added flexibility, the QIAsymphony SP and AS can also be operated independently of each other in independent mode. See "QIAsymphony workflows".

Applications

The QIAsymphony RGQ is the most versatile system for all molecular testing needs — today and tomorrow. The QIAsymphony RGQ allows consolidation of commerical assays, as well as in-house–developed tests, on a single platform.

The broad range of applications for open and closed systems includes:

  • artus transplantation panel (CMV, EBV, HSV, BK virus, and VZV)
  • artus blood-borne virus panel (HIV, HCV, and HBV)
  • In-house tests
Features
Specifications
Altitude Up to 2000 m (6500 ft.)
Elution volume QIAsymphony SP — 30–500 µl (application dependent)
Features Rotor-Gene Q — dynamic range, 10 orders of magnitude
Heat dissipation/thermal load Rotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour)
Humidity QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°CRotor-Gene Q — relative humidity of 10–75% (noncondensing)
Input volume QIAsymphony SP — minimum input volume: 200 µl (application dependent)
Instrument dimensions QIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)
Kits or Application Packs designed for this instrument QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kitsRotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
Operating temperature QIAsymphony SP/AS — 15–32°C (59–89.6ºF)Rotor-Gene Q — 18–30ºC (64–86ºF)
Optical System Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations
Overvoltage category II
Place of operation For indoor use only
Pollution level QIAsymphony SP/AS — 2Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3)
Power QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltagesRotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse
Processing QIAsymphony SP/AS — automated
Protocols/main application on this instrument QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials QIAsymphony AS — PCR setupRotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
Samples per run; throughput QIAsymphony SP — 1–96 samples in batches of 24Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100
Software Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD
Storage temperature QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's packageRotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)
Technology QIAsymphony SP — QIAGEN magnetic-particles chemistryRotor-Gene Q — real-time PCR cycler
Thermal performance Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
Transportation conditions QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's packageRotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)
Typical run time Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
Warranty Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs
Weight QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration)

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Application/Protocol Documents (28)
For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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Instrument User Manuals (7)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with Rotor-Gene Q real-time cyclers
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Rotor-Gene Q User Manual, version 5, for Rotor-Gene Q instruments using Rotor-Gene Q software version 2.3.1, or higher
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For use with QIAsymphony SP/AS instruments (software versions 4.0 and 4.1)
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Instrument Technical Documents (15)
QIAsymphony Cabinet SP/AS documents
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QIAsymphony Cabinet SP/AS document
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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Script change document for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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Differences between QIAsymphony software versions
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Operating Software (5)
Labware package SOW-516-6 for use with the QIAsymphony SP/AS instruments (software version 4.0)
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German language package for QIAsymphony software version 4.1



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QIAsymphony Management Console operating software for QIAsymphony software version 4.0.3
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Differences between QIAsymphony software versions
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QIAsymphony Management Console operating software for QIAsymphony software version 4.1
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Protocol Files (6)
For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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