BioSprint 96 DNA Blood Kit
For rapid and economical automated purification of DNA from a wide range of samples
- Complete removal of contamination and inhibitors
- Purification of high-quality DNA that is ready for use
- Automated procedure saves time and effort
- No organic extraction or alcohol precipitation
The BioSprint 96 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 96.
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Product
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Cat. no.
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List price:
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BioSprint 96 DNA Blood Kit (48)
For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
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940054
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BioSprint 96 DNA Blood Kit (384)
For 384 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
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940057
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The BioSprint 96 DNA Blood Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
BioSprint DNA procedure.|High PCR performance of purified DNA.|Purification of high-quality genomic DNA from mouse tail.|Efficient processing of magnetic particles.|
|Human blood was collected and treated with one of 3 anticoagulants: citrate (C), EDTA (E), or heparin (H). DNA was purified using the BioSprint 96 DNA Blood Kit from 200 µl blood immediately after blood collection (Fresh) and after one cycle of freezing and thawing (Frozen). DNA was eluted in 200 µl elution buffer. Eluates (1 µl and 10 µl) were subjected to PCR (50 µl reaction volume) using primers to amplify a 1 kb fragment of the Hugl gene. PCR products were run on a 1.5% agarose gel in 1x TBE. M: markers.|Mouse tail samples were treated with 180 µl Buffer ATL and 20 µl QIAGEN Proteinase K at 56°C overnight. Genomic DNA was purified from the lysed tissue samples using the BioSprint 96 DNA Blood Kit with the BioSprint 96 DNA Tissue protocol. DNA was eluted in 200 µl elution buffer. Eluates (2 µl) from 8 out of 96 samples were visualized by agarose gel electrophoresis. M: markers.|The BioSprint instrument transfers magnetic particles from one well or tube to another.|
Performance
The BioSprint 96 DNA Blood Kit enables purification of DNA from a variety of sample types (see table).
| Muscle, bovine |
25 mg |
13.5 ± 1.5 |
| Heart, bovine |
25 mg |
3.4 ± 0.6 |
| Spleen, bovine |
25 mg |
59.1 ± 4.8 |
| Lung, bovine |
25 mg |
14.7 ± 5.5 |
| Liver, bovine |
25 mg |
74.0 ± 22.3 |
| Kidney, bovine |
25 mg |
33.5 ± 5.4 |
| Tail, mouse |
1.2 cm (~25 mg) |
30.9 ± 4.5 |
| HL-60 cultured cells |
2 x 106 cells |
9.6 ± 5.6 |
Blood, human
(5–7 x 106 cells per ml) |
200 µl |
4.5–9.0 |
| Blood, bovine |
200 µl |
4.4–7.0 |
| Blood, sheep |
200 µl |
1.2–8.0 |
| Blood, pig |
200 µl |
4.5–9.0 |
| Blood, dog |
200 µl |
6.9–16.1 |
| Blood, cat |
100 µl |
2.9–6.3 |
| Blood, mouse |
100 µl |
1.5–3.0 |
| Blood, rat |
100 µl |
1.0–3.0 |
| Dried blood spots, 903 Specimen Collection Paper |
1 punch (6 mm [1/4 inch] diameter) |
0.10 |
| Dried blood spots, FTA Card |
1 punch (6 mm [1/4 inch] diameter) |
0.05 |
| Buccal swabs |
1 swab |
0.8–2.0 |
Purified DNA is up to 50 kb in size (see figure "Purification of high-quality genomic DNA from mouse tail"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure "High PCR performance of DNA purified using the BioSprint 96 DNA Blood Kit").
Principle
The BioSprint 96 DNA Blood Kit is used with the BioSprint 96. The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. The BioSprint 96 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Procedure
Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
For purification of genomic DNA from tissue, dried blood spots, or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with the BioSprint 96 DNA Blood Kit). We recommend sealing S-Blocks with Tape Pads during sample processing (not needed for rapid blood protocol).
Applications
The BioSprint 96 DNA Blood Kit purifies DNA for use in a range of downstream applications, including:
- Southern blotting
- PCR and real-time PCR
- Genotyping
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Feature
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Specifications
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Applications
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PCR, real-time PCR, genotyping, blotting
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Elution volume
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Custom
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For automated processing
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BioSprint 96 workstation
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Format
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S-Block, 96-well plate
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Main sample type
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Blood, tissue, cells
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Processing
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Automated
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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DNA
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Sample amount
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100–250 µl
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Technology
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Magnetic particles
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Time per run or per prep
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45 minutes
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Yield
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Varies
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For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal swabs, and dried blood spots using the BioSprint 96 workstation
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Show details
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