QIAsymphony Certal Kits, in combination with the QIAsymphony SP, enable fully automated purification of nucleic acids from samples generated during production and purification of cell-culture-based biotechnology-derived products, biopharmaceutical protein drugs (e.g., monoclonal antibodies), or from virus-propagating cell lines for vaccine production. Samples include cell-culture supernatants or fermentation broths after removal of cells, bioprocess purification buffers from chromatography purification procedures, or fractions from vaccine purification (e.g., after inactivation).
The QIAsymphony Certal Residual DNA Kit enables extraction of residual host cell DNA and viral nucleic acids from biologics (e.g., monoclonal antibodies) purified using chromatography procedures. A proprietary buffer (buffer CA) is used to equilibrate matrix effects caused by chromatography reagents, thereby improving the recovery of the nucleic acids from bioprocess samples.
The QIAsymphony Certal Vaccine NA Kit enables extraction of viral nucleic acids from cell-based vaccine production. A proprietary buffer (buffer CB) is used to equilibrate for matrix effects caused by chemical or physical treatment during inactivation and purification of the propagated virus from vaccine production.
Proven, performance-leading magnetic particle technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. The purified nucleic acids are ready for direct use in downstream applications, such as amplification or other detection reactions (e.g., Threshold assay). The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples, in batches of up to 24, are processed in a single run.
For downstream detection, the QIAsymphony Certal Kits are compatible with the Certal CHO Detection Kit and the Certal Vero Detection Kit, enabling highly sensitive detection of residual genomic DNA from CHO and Vero cell lines using quantitative PCR. For high-precision results, we recommend using the Rotor-Gene Q real-time PCR cycler.