artus Enterovirus LC RT-PCR Kit RUO

For quantitative detection of enterovirus RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 3.2 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus Enterovirus LC RT-PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation.
Product Product no. Cat. no. List price:
 
 
show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
Product Cat. no. List price:
artus Enterovirus LC RT-PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
4510003
Log in
artus Enterovirus LC RT-PCR Kit (96) RUO
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
4510005
Log in
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
Reliable quantitation of enterovirus load.|
Detection of the quantitation standards (Enterovirus LC QS 1–4) on LightCycler 1.1/1.2/1.5 instruments using the artus Enterovirus LC RT-PCR Kit. NTC: no template control.|
Performance

To ensure sensitivity, the artus Enterovirus LC RT-PCR Kit has been optimized to detect low numbers of enterovirus RNA. The analytical sensitivity of the kit is 3.2 copies/µl in the PCR.

Performance of artus Enterovirus LC RT-PCR Kit
Kitartus Enterovirus LC RT-PCR Kit
Sample type Cerebral spinal fluid (CSF)
Analytical sensitivity 3.2 copies/µl in the PCR

Principle

The artus Enterovirus LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the enterovirus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus Enterovirus LC RT-PCR Kit provides 4 enterovirus quantitation standards (see figure "Reliable quantitation of enterovirus load". Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical enterovirus RT-PCR is not reduced.

Specifications of artus Enterovirus LC RT-PCR Kit
Kitartus Enterovirus LC RT-PCR Kit
Sample type Cerebral spinal fluid (CSF)
Amplicon 114 bp region of the enterovirus genome

Procedure

The artus Enterovirus LC RT-PCR Kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit.

Applications
The artus Enterovirus LC RT-PCR Kit enables rapid and sensitive detection of enterovirus RNA from cerebrospinal fluid (CSF) with accurate quantification. The kit is available for use on the Lightcycler 1.1/1.2/1.5 Instruments.
Feature
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp Viral RNA Mini Kit
RUO/CE/ASR RUO
Sample type Cerebrospinal fluid (CSF)
Thermal cycler LightCycler
What detected Enterovirus RNA

You are not authorized to download the resource

FAQs
69
FAQ ID -1373
View
FAQ ID -1374
View
FAQ ID -1375
View
FAQ ID -1376
View
FAQ ID -1377
View
FAQ ID -1379
View
FAQ ID -1380
View
FAQ ID -1382
View
FAQ ID -1383
View
FAQ ID -1384
View
FAQ ID -1385
View
FAQ ID -1386
View
FAQ ID -1387
View
FAQ ID -1388
View
FAQ ID -1389
View
FAQ ID -1390
View
FAQ ID -1391
View
FAQ ID -1392
View
FAQ ID -1393
View
FAQ ID -1394
View
FAQ ID -1395
View
FAQ ID -1396
View
FAQ ID -1397
View
FAQ ID -1398
View
FAQ ID -1399
View
FAQ ID -1400
View
FAQ ID -1401
View
FAQ ID -1486
View
FAQ ID -1487
View
FAQ ID -1488
View
FAQ ID -1489
View
FAQ ID -1490
View
FAQ ID -1491
View
FAQ ID -1492
View
FAQ ID -1493
View
FAQ ID -1494
View
FAQ ID -1495
View
FAQ ID -1496
View
FAQ ID -1497
View
FAQ ID -1498
View
FAQ ID -1499
View
FAQ ID -1500
View
FAQ ID -1501
View
FAQ ID -1502
View
FAQ ID -1503
View
FAQ ID -1504
View
FAQ ID -1505
View
FAQ ID -1506
View
FAQ ID -1507
View
FAQ ID -1508
View
FAQ ID -1509
View
FAQ ID -1510
View
FAQ ID -1511
View
FAQ ID -1512
View
FAQ ID -1514
View
FAQ ID -1515
View
FAQ ID -1516
View
FAQ ID -1517
View
FAQ ID -1518
View
FAQ ID -1520
View
FAQ ID -1521
View
FAQ ID -1522
View
FAQ ID -1523
View
FAQ ID -1524
View
FAQ ID -1525
View
FAQ ID -1527
View
FAQ ID -1529
View
FAQ ID -1692
View
FAQ ID -1528
View