GeneRead DNAseq Targeted Panels V2

For targeted enrichment of biologically and clinically relevant panels using any of 15 laboratory-verified primer sets prior to NGS
  • Outstanding sequencing performance
  • As little as 10 ng DNA needed
  • Compatible with many samples types including FFPE samples
  • Can be used on any sequencing platform
  • Clinically and biologically focused content
GeneRead DNAseq Targeted Panels V2 are the simplest tools for analyzing the genetic variants of a focused panel of genes via next-generation sequencing. Each panel consists of multiplex PCR primer sets to amplify exonic regions of a thoroughly researched panel of biologically and clinically relevant and disease-focused genes. GeneRead DNAseq Targeted Panels V2 can also be customized to include genes or other genomic regions tailored to your specific NGS project needs. Our high-quality primer design and targeted enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples.
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Available Products
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Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-001X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-002X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-003X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-004X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-005X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-006X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-007X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-008X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-009X varies
Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181901 NGHS-011X varies
Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181901 NGHS-013X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-101X varies
Sets of 4 pools containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
show details
181900 NGHS-102X varies
Sets of 1 tube containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181900 NGHS-201X varies
Sets of 4 tubes containing wet-bench verified primer sets for targeted exon enrichment of a pathway-focused panel of genes
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181901 NGHS-501X varies
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GeneRead DNAseq Targeted Panels V2 are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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GeneRead DNAseq Targeted Panel V2 workflow.|Multiplex PCR-enabled target enrichment of genomic regions of interest|
Genomic DNA (the QIAamp DNA Mini Kit, QIAamp DNA FFPE Tissue Kit, or GeneRead DNA FFPE Kit are recommended for extraction) can be quantified and qualified using the GeneRead DNA QuantiMIZE System. The GeneRead DNAseq Targeted Panels V2 are then used for targeted enrichment. Following target enrichment, construct your NGS library, quantify and quality-control using the GeneRead Library Quantification System, perform NGS, and analyze the data using the QIAGEN NGS Data Analysis Web Portal.|The principle of the GeneRead DNAseq V2 System is to employ overlapping primer sets across the exonic portions of a gene or genes to maximize target coverage and minimize nonspecific amplification.|
Performance
The performance of GeneRead DNAseq Targeted Panels V2 is assessed by 3 criteria: design coverage, specificity, and coverage uniformity. Greater than 95% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 95% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.

Outstanding experimental performance metrics
 Type  Panel name Coverage (%)  Specificity (%)  Uniformity (%)
 Solid tumors  Tumor Actionable Mutations  100.0  98.2  91
 Clinically Relevant Tumor  98.1  95.3  90
 Hematologic malignancies  Myeloid Neoplasms   98.1  97.4  94
 Disease-specific  Breast Cancer  98.2  96.8  91
 Colorectal Cancer  98.7  98.3  95
 Liver Cancer  99.0  96.4  96
 Lung Cancer  97.5  98.1  90
 Ovarian Cancer  98.9  96.6  96
 Prostate Cancer  98.4  97.3  94
 Gastric Cancer  98.1  98.5  93
 Cardiomyopathy  96.3  96.7  87
 Comprehensive  Carrier Testing  97.5  97.9  91
 Cancer Predisposition  98.3  96.8  93
 Comprehensive Cancer  98.0  97.7  92
 Gene-specific  BRCA1 and BRCA2
 100.0  99.0  97
Principle
The GeneRead DNAseq Targeted Panel System employs overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see Multiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into an appropriate number of pools to maximize specificity. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.

Specifications of GeneRead DNAseq Targeted Panels V2
 Type  Panel name # Genes  # Amplicons  Target region (bases)
 Solid tumors  Tumor Actionable Mutations  8  118  7104
Clinically Relevant Tumor  24  602  39603
 Hematologic malignancy  Myeloid Neoplasms  50  2536  236319
 Disease-specific  Breast Cancer  44  2915  268621
 Colorectal Cancer  38  1954  182851
 Liver Cancer  33  2052  191170
 Lung Cancer  45  3586  332999
 Ovarian Cancer  32  2021  198058
 Prostate Cancer  32  1837  167195
 Gastric Cancer  29  2377  222333
 Cardiomyopathy  58  2657  249727
 Comprehensive  Carrier Testing  157  6943  664735
 Cancer Predisposition  143  6582  620318
 Comprehensive Cancer  160  7951  744835
 Gene-specific  BRCA1 and BRCA2
 2  250
 21472
Procedure
GeneRead DNAseq Targeted Panels V2 are part of a total workflow for targeted next-generation sequencing (see GeneRead DNAseq Targeted Panels V2 workflow). Simply extract DNA from your samples (the QIAamp DNA Mini Kit, the QIAamp DNA FFPE Tissue Kit or the GeneRead DNA FFPE Kit are recommended), quantify and qualify your DNA sample with the GeneRead DNA QuantiMIZE system, and then use the GeneRead DNAseq Targeted Panels V2 in combination with GeneRead DNAseq Panel PCR Kit V2 to perform targeted enrichment using multiplex PCR. Once targets have been enriched, construct the NGS library and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Perform NGS. Multiple samples can be analyzed with a single NGS run. Use the Multiplexing Capacity Calculator (see the Resources tab) to determine the optimal number of samples and coverage depth for each NGS run. Analyze your data using the QIAGEN NGS Data Analysis Web Portal or CLC Bio pipelines, and interpret detected variants using the Ingenuity Variant Analysis platform.
Applications
GeneRead DNAseq Targeted Panels V2 are highly suited for enriching target genes specific for various cancers, inherited diseases, neurological diseases, cardiomyopathy, developmental disorders, metabolic disorders and many other areas of biomedical research to determine genetic variants through NGS.

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Brochures & Guides
2
GeneRead Panels and NGS product configurator guide
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For targeted enrichment prior to next-generation sequencing
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Kit Handbooks
1
All-bead protocol for targeted enrichment prior to next-generation sequencing
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Analysis Software
3
For analyzing, comparing, and visualizing next-generation sequencing data
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For basic analysis of NGS data using the GeneRead DNAseq panels V2; acceptable file formats include FASTQ or BAM
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For identifying disease-causing variants from human whole genome, exome, and gene panel next-generation sequencing studies
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