Have you tested the effect of inhibitors on PCR performance?
FAQ ID -818
Yes. Please see Table 3 in our brochure Maximizing PCR and RT-PCR success. We tested the effects of different inhibitory substances in a number of PCR systems. We also analyzed the effect of including different volumes of reverse transcription (RT) reaction mixtures in PCR. Please see the table below for a list of commonly encountered template impurities and their inhibitory effects on PCR.
Impurities showing inhibitory effects on PCR
| Substance |
Inhibitory concentration |
| SDS |
>0.005% (w/v) |
| Phenol |
>0.2% (v/v) |
| Ethanol |
>1% (v/v) |
| Isopropanol |
>1% (v/v) |
| Sodium Acetate |
≥5 mM |
| Sodium Chloride |
≥25 nM |
| EDTA |
≥0.5 mM |
| Hemoglobin |
≥1 mg/ml |
| Heparin |
≥0.15 i.U./ml |
| Urea |
>20 mM |
| RT reaction mixture |
≥15% |