The importance of the miRNA regulatory pathways is underscored by the impressive list of diseases that have recently been found to be associated with abnormal miRNA expression. These include cancer, age-related diseases, heart disease, neurological diseases, and immune function disorders.
This webinar introduces a simple and easy-to-use real-time PCR method to individually study miRNA expression or to examine genomewide, pathway-focused or disease-focused miRNA expression profiles. This method enables any lab with a real-time PCR instrument to explore the miRNA world. The webinar also describes how to purify small RNA from cells and tissues, as well as challenging sample types such as serum and formalin-fixed tissue samples.
For further study, the webinar details tools for functional analysis such as miRNA mimics, inhibitors, and target protectors, which are commonly used to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs. In addition, miRNA target protectors provide valuable insights into the roles of miRNAs in regulation of individual genes.
The following topics will be discussed:
- miRNA research trends
- miRNA purification from various samples — challenges and solutions
- miRNA expression profiling using miRNA PCR arrays
- Functional analysis of miRNA
- Q&A session (15 min)