Advantages of Capillary Electrophoresis-based Analysis in Molecular Microbiology


Bacterial and viral organisms are the main topic of interest for many labs and play an important role in a number of application areas such as infectious disease research, microbiology, immunology, food inspection, and environmental research.

The spectrum of methods used to study microbial organisms has dramatically changed over the last century, from observation and cultivation in the mid-20th century to a large range of nucleic acid-based molecular methods, among which PCR-based analysis plays a major role. The most commonly used method for readout of end-point PCR-based assays is gel electrophoresis, using manually poured slab gels. This method is highly labor-intensive, resolution of such gels is often poor, and thorough analysis of the data in terms of fragment size can be tedious - particularly if the data are to be compared with previously analyzed PCR products.

The following topics will be discussed in this one-hour webinar:

The fundamentals of capillary electrophoresis technology for read-out of PCR assays as performed on QIAGEN's QIAxcel system
The advantages of automated capillary electrophoresis in comparison to slab gels with respect to resolution, speed, workflow standardization, data analysis, and documentation
Data will be presented from molecular applications for detection of antibiotic resistance genes and species identification

Anna Ryberg

Anna Ryber is a molecular biologist at the Department of Clinical Microbiology, Center of Diagnostics, University Hospital, Linkköping, Sweden.

Anna studied for a Master of Science in Biology/Biotechnology at the Swedish University of Agricultural Sciences (SLU) in Uppsala. Since 2010, Anna has been developing molecular analyses for routine detection of antibiotic-resistant bacterial genes and molecular epidemiology tools for infection control.
Dr. Carola Schade

Carola Schade
Dr. Carola Schade joined QIAGEN in 1999. She is involved in developing automated solutions for sample purification and analysis and introducing them to the global marketplace. She currently holds the position of Director, Head of Instruments Business Life Science. Carola studied at the Heinrich-Heine University of Düsseldorf where she obtained her PhD in Biology at the Institute for Molecular Genetics.