Using High-Resolution Melting analysis and Pyrosequencing to identify and characterize microorganisms


High-Resolution Melting (HRM) analysis and Pyrosequencing have excellent applications in the identification and characterization of microorganisms. Information about antibiotic resistance genes and species identification can be obtained rapidly and with greater certainty using the instruments that are now on the market. The presentation includes an overview of HRM technology on QIAGEN's Rotor-Gene Q cycler, and shows how it can be used with Pyrosequencing. The focus will be on three research projects:

Aspergillus fumigatus Azole resistance detection and confirmation
Microorganism identification using Pyrosequencing and HRM
SNP genotyping of the Toll-like receptor

Marion Tuohy

Marion Tuohy is an MT(ASCP) who has worked in the Microbiology Research Laboratory at Cleveland Clinic for the past fourteen years. She has worked with Pyrosequencing since 2001 with an emphasis on its clinical applications. Rapid and accurate diagnosis of clinical infections is the current focus of the laboratory's molecular research.