Back to top Principle
Extraction of nucleic acids is a first, crucial step for many DNA and RNA analysis methods. However, this process can be time-consuming, resource intensive, and often requires expensive laboratory equipment. The SpeedXtract Nucleic Acid Kit is specifically designed to offer fast and reliable extraction of nucleic acids using a convenient, centrifugation-free procedure. The procedure is therefore particularly useful for point-of-need molecular testing in locations without sophisticated laboratory infrastructure (e.g., remote areas).
Back to topProcedure
The SpeedXtract procedure uses powerful heat lysis to lyse cells, optimized buffers to stabilize extracted nucleic acids, and high-performance magnetic-particle technology to remove contaminants and cell debris (see figure Convenient, manual nucleic acid extraction procedure for swab and liquid samples). The efficient protocol allows extraction of nucleic acids from a swab sample in as little as 7 minutes, with only one minute of hands-on time (see table). Liquid samples can be processed in as little as 12 minutes, with only 2 minutes of hands-on time.
Back to topApplications
|Sample source types
||Swabs or pre-concentrated cells
||Liquid samples, e.g., urine or whole blood
|Target nucleic acids
||Bacterial, eukaryotic, and viral DNA
||Bacterial, eukaryotic, and viral DNA
|Preparation time per sample
||Approx. 7 min (incl. 1 min hands-on time)*
||Approx. 12 min (incl. 2 min hands-on time)*†
The SpeedXtract Nucleic Acid Kit is ideally suited for fast and reliable extraction of nucleic acids. As the procedure is centrifugation free, it is particularly useful for point-of-need testing in locations without sophisticated laboratory infrastructure (e.g., remote areas).
Efficient removal of contaminants using SpeedXtract magnetic particles
The magnetic particles used in the SpeedXtract Nucleic Acid Kit procedure effectively remove contaminants and substances that can inhibit downstream DNA analysis applications (see figure Magnetic particle separation efficiently removes contaminants). Nasal swab samples from healthy volunteers were spiked with Gram-positive bacteria and subjected to the SpeedXtract nucleic acid extraction procedure for swab samples, either including or excluding magnetic-particle based contaminant removal. The inclusion of magnetic particle-based contaminant removal resulted in shorter amplification times in an isothermal amplification assay, indicating that potential nucleic acid amplification inhibitors were successfully removed by this process.
Reproducible extraction of nucleic acids from urine
The SpeedXtract Nucleic Acid Kit efficiently and reproducibly extracts nucleic acids from urine, which can contain high amounts of inhibitory substances, salts, and nucleases, with high variability between subjects (see figure Reproducible nucleic acid extraction from urine). Urine samples from healthy volunteers were spiked with Gram-negative bacteria and subjected to the SpeedXtract nucleic acid extraction procedure. Highly similar CT values from real-time PCR amplification demonstrate the reliability of the extraction procedure.
Reliable extraction of DNA from bacterial cells in blood
Data indicates that SpeedXtract Nucleic Acid Kit provides reliable extraction of bacterial DNA from both low- and high-abundance cells in blood (see figure Extraction of bacterial DNA from low- and high-abundance cells in blood). Whole blood samples from 5 healthy volunteers were mixed and aliquots were spiked with Gram-positive bacteria at different concentrations. Samples were subjected to the SpeedXtract nucleic acid extraction procedure. CT values from real-time PCR amplification showed a linear relationship with bacterial concentration, demonstrating that the SpeedXtract Nucleic Acid Kit provides reliable extraction of bacterial DNA over a broad linear range. CT values of internal control (IC) DNA in the presence of SpeedXtract nucleic acid extracts were highly similar to those for IC DNA in the presence of water, indicating that the SpeedXtract nucleic acid extraction procedure successfully removed PCR inhibitors.
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