Expression of β-actin (cytoskeleton protein) was analyzed by real-time one-step RT-PCR. The FastLane Kit provided CT values comparable to those achieved with the RNeasy Mini Kit and QuantiTect Probe RT-PCR Kit.
The FastLane Kit gave lower CT values in real-time one-step RT-PCR than similar kits from other suppliers. Each kit was used with an assay for PPIA (an isomerase).
Real-time one-step RT-PCR analysis of the expression of NFκB1 (nuclear transcription factor) using the FastLane Kit gave reproducible CT values from a 384-well plate of HeLa cells.
The FastLane Kit enabled linear detection of β-actin transcript in 1–10,000 HeLa cells (serial dilutions of lysate were analyzed). In contrast, a similar kit from Supplier SIII showed no linearity.